Research Article
Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis
Table 1
Prime sequences for quantitative real-time PCR.
| Gene name | Sequence (5 to 3) |
| Rat IL-6 sense | ACCACCCACAACAGACCAGT | Rat IL-6 antisense | ACAGTGCATCATTCGCTGTTC | Rat TNF-α sense | AAATGGGCTCCCTCTCATCAGTTC | Rat TNF-α antisense | TCTGCTTGGTGGTTTGCTACGAC | Rat HIF-α sense | GCTGCCTCTTCGACAAGCTT | Rat HIF-α antisense | CGCTGGAGCTAGCAGAGTCA | Rat β-actin sense | CACCCGCGAGTACAACCTTC | Rat β-actin antisense | CCCATACCCACCATCACACC |
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