Research Article

Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis

Table 1

Prime sequences for quantitative real-time PCR.

Gene nameSequence (5 to 3)

Rat IL-6 senseACCACCCACAACAGACCAGT
Rat IL-6 antisenseACAGTGCATCATTCGCTGTTC
Rat TNF-α senseAAATGGGCTCCCTCTCATCAGTTC
Rat TNF-α antisenseTCTGCTTGGTGGTTTGCTACGAC
Rat HIF-α senseGCTGCCTCTTCGACAAGCTT
Rat HIF-α antisenseCGCTGGAGCTAGCAGAGTCA
Rat β-actin senseCACCCGCGAGTACAACCTTC
Rat β-actin antisenseCCCATACCCACCATCACACC