[Retracted] lncRNA GHET1 Promotes the Progression of Triple-Negative Breast Cancer via Regulation of miR-377-3p/GRSF1 Signaling Axis

Figure 5

GRSF1 is a downstream target of miR-377-3p. (a) Target gene of miR-377-3p was predicted by the TargetScan database (up panel). Interaction between miR-377-3p and GRSF1 was analyzed by dual-luciferase reporter gene, vs. NC mimics. (b) GRSF1 expression was detected by qRT-PCR in TBNC tissues, vs. the normal group. (c) miR-377-3p expression was detected by qRT-PCR in the indicated TNBC cells. (d) GRSF1 protein expression was detected by Western blotting in the indicated TNBC cells. (e) GRSF1 mRNA expression was detected by qRT-PCR in the miR-377-3p mimic- or inhibitor-transfected TNBC cells, vs. NC mimics/NC inhibitor. (f) Correlation between miR-377-3p and lncRNAGRSF1 in TNBC tissues was analyzed by Pearson correlation. (g) qRT-PCR was used to detect GRSF1 expression in the TNBC cells overexpressed or knocked down lncRNA GHET1, vs. the vector/sh-NC group. (h) Correlation between GRSF1 and lncRNA GHET1 in TNBC tissues was analyzed by Pearson correlation analysis. Each data was the average of three independent assays.

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