Research Article

[Retracted] HD-13 Induces Swine Pneumonia Progression via Activation of TLR9

Figure 2

HD-13 activates the inflammatory response via accelerating TLR9 expression. (a, b) RT-qPCR was performed to assess the mRNA levels of TLR9, MyD88, TRIF, and TRAF6. (c) Western blot was conducted to evaluate the levels of MyD88, TRIF, and TRAF6 proteins. (d) RT-qPCR was carried out to detect the mRNA levels of IL-1β, IL-6, IL-12, and TNF-α. (e) ELISA was performed to detect the expression of IL-1β, IL-6, IL-12, and TNF-α in the supernatant of 3D4/21 cells. vs. control group, ###vs. control group, and $$vs. HD-13 group.
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