[Retracted] HD-13 Induces Swine Pneumonia Progression via Activation of TLR9

Figure 4

HD-13 activates MAPK and NF-κB pathways via accelerating TLR9 expression. 3D4/21 cells were randomly divided into 4 groups: Scra group—cells were transfected with Scra siRNA for 48 h, which serves as a negative control; TLR9 siRNA group—cells were transfected with TLR9 siRNA for TLR9 silencing for 48 h; HD-13+Scra group—cells were infected with HD-13 for 48 h; and HD-13+TLR9 siRNA group—cells were infected with HD-13 and transfected with TLR9 siRNA for TLR9 silencing for 48 h. (a) Western blot was conducted to evaluate the levels of MAPK pathway-related proteins, including ERK, p-ERK, p38, p-p38, JNK, and p-JNK. (b) The levels of NF-κB pathway-related proteins, including p65, p-p65, IKKα, and p-IKKα, were measured by Western blot. (c) Immunofluorescence was performed to assess p-p65 expression. , vs. control group; ^##, ^###vs. control group; ^$, ^$$vs. HD-13 group.

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