[Retracted] Heme Oxygenase-1 Inhibits the Proliferation of Hepatic Stellate Cells by Activating PPARγ and Suppressing NF-κB

Figure 1

Selection for the most appropriate concentrations and incubation periods for Hemin and ZnPP-IX. (a) The effects of the different concentrations of Hemin and ZnPP-IX on HSC-T6 proliferation were assessed by the MTT assay. Hemin suppressed HSC proliferation in a dose-dependent manner, which was significant at 24 hours (). Moreover, ZnPP-IX dose-dependently promoted HSC proliferation, which was significant at 24 hours (). (b) LDH level in cultured HSC-T6 cells exposed to Hemin or ZnPP-IX. H-A, H-B, and H-C: 6.5, 13, and 26 μg/ml Hemin, respectively; Z-A, Z-B, and Z-C: 3, 6, and 12 μg/ml ZnPP-IX, respectively. There were no significant differences in LDH release from the cells treated with 6.5 and 13 μg/ml Hemin for 12 or 24 hours relative to the control (, , , and , respectively). However, LDH release from the cells treated with 6.5 or 13 μg/ml Hemin for 48 hours or with 26 μg/ml Hemin for all time points was notably enhanced compared to that from the normal (). For the ZnPP-IX-treated group, the LDH release levels from the cells treated with 12 μg/ml ZnPP-IX for 24 hours and with all concentrations for 48 hours were significantly elevated as compared to those for the normal ().

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