Circular RNA_HIPK3-Targeting miR-93-5p Regulates KLF9 Expression Level to Control Acute Kidney Injury

Figure 1

circHIPK3 is upregulated in acute kidney injury. (a) The level of serum CREA, BUN, and uric acid in I/R and sham groups. (b) Representative H&E images are shown in I/R and sham groups. (c) The level of IL-1β, IL-6, and TNF-α was detected via ELISA. (d) The level of apoptosis-associated protein (Bcl2, Bax, and Cyt-c) was measured via western blot. (e) The level of circHIPK3 was assessed in the I/R and sham groups. (f) H/R treatment was performed in the HK-2 cell, and the level of IL-1β, IL-6, and TNF-α was detected via ELISA. (g) The level of apoptosis-associated protein was measured via western blot. (h). Cell viability was detected via MTT. (i) The production of ROS was evaluated via the Reactive Oxygen Species Assay Kit. (j) The content of ATP was measured via the ATP Assay Kit. (k) The level of circHIPK3 was assessed in the H/R and control groups. and vs. Control. The experiments were repeated at least three times. The results were expressed as .

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