Circular RNA_HIPK3-Targeting miR-93-5p Regulates KLF9 Expression Level to Control Acute Kidney Injury

Figure 3

circHIPK3 interacting with miR-93-5p regulates AKI progression. (a) Schematic diagram of bioinformatics prediction binding sites (upper). Luciferase assay report for circHIPK3 and miR-93-5p (lower). (b, c) The level of miR-93-5p was measured in vivo and in vitro. (d) The level of circHIPK3 and miR-93-5p was detected via qRT-PCR. (e) Cell viability was measured in different groups. (f) The level of IL-1β, IL-6, and TNF-α was detected via ELISA. (g) The production of ROS was evaluated. (h) The content of ATP was measured via ATP Assay Kit. (i) The apoptosis rate of the HK-2 cell after different treatments was detected via flow cytometry. vs. Control group, ^# vs. H/R group, and ^& vs. H/R+circHIPK3 group. The experiments were repeated at least three times. The results were expressed as .

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