Circular RNA_HIPK3-Targeting miR-93-5p Regulates KLF9 Expression Level to Control Acute Kidney Injury

Figure 4

KLF9 is a target of miR-93-5p. (a) Schematic diagram of bioinformatics prediction binding sites (upper). Luciferase assay report for KLF9 and miR-93-5p (lower). (b, c) The mRNA level of KLF9 was measured in vivo and in vitro. (d, e) The protein level of KLF9 was measured in vivo and in vitro. (f) The level of miR-93-5p and KLF9 was detected via qRT-PCR. (g) Cell viability was measured in different groups. (h) The production of ROS was evaluated. (i) The content of ATP was measured via ATP Assay Kit. (j) The apoptosis rate of HK-2 cell after different treatments was detected via flow cytometry. and vs. Control group, ^# vs. H/R group, ^& vs. H/R+KLF9 group, and ^& vs. H/R+KLF9 group. The experiments were repeated at least three times. The results were expressed as .

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