Circular RNA_HIPK3-Targeting miR-93-5p Regulates KLF9 Expression Level to Control Acute Kidney Injury

Figure 5

circHIPK3 is implicated in AKI via regulating the miR-93-5p/KLF9 axis. (a) AAV9 vector plasmid was injected into mice before operation. The level of circHIPK3, miR-93-5p, and KLF9 was detected via qRT-PCR. (b) The level of serum CREA, BUN, and uric acid in different groups. (c) Representative H&E images are shown in different groups. The section shows acute tubular necrosis characterized by loss of tubular epithelial cells. (d) Representative TUNEL staining images are shown in different groups. (e) The level of IL-1β, IL-6, and TNF-α was detected via ELISA. (f) The level of apoptosis-associated proteins (Bcl2, Bax, and Cyt-c) was measured via western blot. and vs. Sham group, ^# vs. I/R group, and ^& vs. I/R+circHIPK3 group. The experiments were repeated at least three times. The results were expressed as .

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