Optimal Design of Single-Cell Experiments within Temporally Fluctuating Environments

Figure 1

Stochastic modeling of osmotic stress response genes in yeast. (a) Four-state model of gene expression, where each state transcribes mRNA at a different transcription rate, but each mRNA degrades at a single rate γ. (b) Time-varying MAPK nuclear localization signal. (c) The rate of switching from gene activation state S2 to S1 (right) under 0.2 M or 0.4 M NaCl osmotic stress. The time at which k₂₁ turns off is denoted with τ₁ and is independent of the NaCl level. The time at which k₂₃ turns back on is given by τ_NaCl depending on the level of NaCl. (d) Time evolution of the STL1 mRNA in response to the 0.2 M and 0.4 M NaCl stress. Model and parameters from [10] are summarized in Supplementary Notes I and II and Supplementary Tables I and II.

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