Optimal Design of Single-Cell Experiments within Temporally Fluctuating Environments

Figure 2

Verification of the FSP-FIM for the time-varying HOG1-MAPK model. (a) Marginal parameter histograms (top panels) and joint scatter plots (gray dots) for the MLE parameter estimates from 50 simulated datasets and for a subset of model parameters. All parameters are shown in logarithmic scale. The ellipses show the 95% CI for the inverse of the FIM (purple) and Gaussian approximation of MLE scatter plot (orange). The yellow dots indicate the “true” parameters at which the FIM and simulated datasets were generated. (b) Rank-paired eigenvalues for the covariance of MLE estimates (orange) and inverse of the FIM (blue). The angles between corresponding rank-paired eigenvectors are shown in degrees.

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