Table of Contents
Cardiovascular Psychiatry and Neurology
Volume 2011 (2011), Article ID 646958, 9 pages
http://dx.doi.org/10.1155/2011/646958
Research Article

Slice Cultures as a Model to Study Neurovascular Coupling and Blood Brain Barrier In Vitro

Institute for Neurophysiology, Charité-Universitätsmedizin Berlin, Oudenarder Strasse 16, 13347 Berlin, Germany

Received 30 September 2010; Accepted 24 December 2010

Academic Editor: Alon Friedman

Copyright © 2011 Richard Kovács et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Banerjee and M. A. Bhat, “Neuron-glial interactions in blood-brain barrier formation,” Annual Review of Neuroscience, vol. 30, pp. 235–258, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. M. Zonta, M. C. Angulo, S. Gobbo et al., “Neuron-to-astrocyte signaling is central to the dynamic control of brain microcirculation,” Nature Neuroscience, vol. 6, no. 1, pp. 43–50, 2003. View at Publisher · View at Google Scholar · View at Scopus
  3. C. Iadecola, “Neurovascular regulation in the normal brain and in Alzheimer's disease,” Nature Reviews Neuroscience, vol. 5, no. 5, pp. 347–360, 2004. View at Google Scholar · View at Scopus
  4. S. J. Mulligan and B. A. MacVicar, “Calcium transients in astrocyte endfeet cause cerebrovascular constrictions,” Nature, vol. 431, no. 7005, pp. 195–199, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. J. A. Filosa, A. D. Bonev, S. V. Straub et al., “Local potassium signaling couples neuronal activity to vasodilation in the brain,” Nature Neuroscience, vol. 9, no. 11, pp. 1397–1403, 2006. View at Publisher · View at Google Scholar · View at Scopus
  6. E. Seiffert, J. P. Dreier, S. Ivens et al., “Lasting blood-brain barrier disruption induces epileptic focus in the rat somatosensory cortex,” Journal of Neuroscience, vol. 24, no. 36, pp. 7829–7836, 2004. View at Publisher · View at Google Scholar · View at Scopus
  7. Y. David, L. P. Cacheaux, S. Ivens et al., “Astrocytic dysfunction in epileptogenesis: consequence of altered potassium and glutamate homeostasis?” Journal of Neuroscience, vol. 29, no. 34, pp. 10588–10599, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. H. Girouard and C. Iadecola, “Neurovascular coupling in the normal brain and in hypertension, stroke, and Alzheimer disease,” Journal of Applied Physiology, vol. 100, no. 1, pp. 328–335, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. J. A. Filosa, “Vascular tone and neurovascular coupling: considerations toward an improved in vitro model,” Front Neuroenergetics, vol. 2, article 16, 2010. View at Publisher · View at Google Scholar
  10. M. Gumbleton and K. L. Audus, “Progress and limitations in the use of in vitro cell cultures to serve as a permeability screen for the blood-brain barrier,” Journal of Pharmaceutical Sciences, vol. 90, no. 11, pp. 1681–1698, 2001. View at Publisher · View at Google Scholar · View at Scopus
  11. B. H. Gahwiler and F. Hefti, “Guidance of acetylcholinesterase-containing fibres by target tissue in co-cultured brain slices,” Neuroscience, vol. 13, no. 3, pp. 681–689, 1984. View at Publisher · View at Google Scholar · View at Scopus
  12. M. Frothscher and B. H. Gahwiler, “Synaptic organization of intracellularly stained CA3 pyramidal neurons in slice cultures of rat hippocampus,” Neuroscience, vol. 24, no. 2, pp. 541–551, 1988. View at Google Scholar · View at Scopus
  13. L. Stoppini, P. A. Buchs, and D. Muller, “A simple method for organotypic cultures of nervous tissue,” Journal of Neuroscience Methods, vol. 37, no. 2, pp. 173–182, 1991. View at Publisher · View at Google Scholar · View at Scopus
  14. S. Duport, F. Robert, D. Muller, G. Grau, L. Parisi, and L. Stoppini, “An in vitro blood-brain barrier model: cocultures between endothelial cells and organotypic brain slice cultures,” Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 4, pp. 1840–1845, 1998. View at Publisher · View at Google Scholar · View at Scopus
  15. C. M. Zehendner, H. J. Luhmann, and C. R. W. Kuhlmann, “Studying the neurovascular unit: an improved blood-brain barrier model,” Journal of Cerebral Blood Flow and Metabolism, vol. 29, no. 12, pp. 1879–1884, 2009. View at Publisher · View at Google Scholar · View at Scopus
  16. K. V. Moser, R. Schmidt-Kastner, H. Hinterhuber, and C. Humpel, “Brain capillaries and cholinergic neurons persist in organotypic brain slices in the absence of blood flow,” European Journal of Neuroscience, vol. 18, no. 1, pp. 85–94, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. K. Bendfeldt, V. Radojevic, J. Kapfhammer, and C. Nitsch, “Basic fibroblast growth factor modulates density of blood vessels and preserves tight junctions in organotypic cortical cultures of mice: a new in vitro model of the blood-brain barrier,” Journal of Neuroscience, vol. 27, no. 12, pp. 3260–3267, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. R. S. Camenzind, S. Chip, H. Gutmann, J. P. Kapfhammer, C. Nitsch, and K. Bendfeldt, “Preservation of transendothelial glucose transporter 1 and P-glycoprotein transporters in a cortical slice culture model of the blood-brain barrier,” Neuroscience, vol. 170, no. 1, pp. 361–371, 2010. View at Publisher · View at Google Scholar
  19. R. Kovács, R. Gutiérrez, A. Kivi, S. Schuchmann, S. Gabriel, and U. Heinemann, “Acute cell damage after low Mg-induced epileptiform activity in organotypic hippocampal slice cultures,” NeuroReport, vol. 10, no. 2, pp. 207–213, 1999. View at Google Scholar · View at Scopus
  20. R. Kovács, A. Rabanus, J. Otáhal et al., “Endogenous nitric oxide is a key promoting factor for initiation of seizure-like events in hippocampal and entorhinal cortex slices,” Journal of Neuroscience, vol. 29, no. 26, pp. 8565–8577, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. S. Bolte and F. P. Cordelières, “A guided tour into subcellular colocalization analysis in light microscopy,” Journal of Microscopy, vol. 224, no. 3, pp. 213–232, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Andreasen and G. Danscher, “Optical slicing and 3-D characterization of hippocampal capillaries in the rat visualized by autometallographic silver enhancement of colloidal gold particles,” Histochemical Journal, vol. 29, no. 10, pp. 775–781, 1997. View at Publisher · View at Google Scholar · View at Scopus
  23. R. Kovács, J. Kardos, U. Heinemann, and O. Kann, “Mitochondrial calcium ion and membrane potential transients follow the pattern of epileptiform discharges in hippocampal slice cultures,” Journal of Neuroscience, vol. 25, no. 17, pp. 4260–4269, 2005. View at Publisher · View at Google Scholar · View at Scopus
  24. K. M. Robinson, M. S. Janes, and J. S. Beckman, “The selective detection of mitochondrial superoxide by live cell imaging,” Nature Protocols, vol. 3, no. 6, pp. 941–947, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. K. Jandová, D. Päsler, L. L. Antonio et al., “Carbamazepine-resistance in the epileptic dentate gyrus of human hippocampal slices,” Brain, vol. 129, no. 12, pp. 3290–3306, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. I. Manzini and D. Schild, “Multidrug resistance transporters in the olfactory receptor neurons of Xenopus laevis tadpoles,” Journal of Physiology, vol. 546, no. 2, pp. 375–385, 2003. View at Publisher · View at Google Scholar · View at Scopus
  27. G. R. J. Gordon, H. B. Choi, R. L. Rungta, G. C. R. Ellis-Davies, and B. A. MacVicar, “Brain metabolism dictates the polarity of astrocyte control over arterioles,” Nature, vol. 456, no. 7223, pp. 745–750, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. C. Capone, G. Faraco, J. Anrather, P. Zhou, and C. Iadecola, “Cyclooxygenase 1-derived prostaglandin E2 and EP1 receptors are required for the cerebrovascular dysfunction induced by angiotensin II,” Hypertension, vol. 55, no. 4, pp. 911–917, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. M. Yemisci, Y. Gursoy-Ozdemir, A. Vural, A. Can, K. Topalkara, and T. Dalkara, “Pericyte contraction induced by oxidative-nitrative stress impairs capillary reflow despite successful opening of an occluded cerebral artery,” Nature Medicine, vol. 15, no. 9, pp. 1031–1037, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. J. Noraberg, F. R. Poulsen, M. Blaabjerg et al., “Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair,” Current Drug Targets: CNS and Neurological Disorders, vol. 4, no. 4, pp. 435–452, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. B. Lassègue and R. E. Clempus, “Vascular NAD(P)H oxidases: specific features, expression, and regulation,” American Journal of Physiology, vol. 285, no. 2, pp. R277–R297, 2003. View at Google Scholar · View at Scopus
  32. M. Dai, A. Nuttall, Y. Yang, and X. Shi, “Visualization and contractile activity of cochlear pericytes in the capillaries of the spiral ligament,” Hearing Research, vol. 254, no. 1-2, pp. 100–107, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. P. Ballabh, A. Braun, and M. Nedergaard, “The blood-brain barrier: an overview: structure, regulation, and clinical implications,” Neurobiology of Disease, vol. 16, no. 1, pp. 1–13, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. H. Shalev, Y. Serlin, and A. Friedman, “Breaching the blood-brain barrier as a gate to psychiatric disorder,” Cardiovascular Psychiatry and Neurology, vol. 2009, Article ID 278531, 7 pages, 2009. View at Publisher · View at Google Scholar
  35. X. E. Ndode-Ekane, N. Hayward, O. Gröhn, and A. Pitkänen, “Vascular changes in epilepsy: functional consequences and association with network plasticity in pilocarpine-induced experimental epilepsy,” Neuroscience, vol. 166, no. 1, pp. 312–332, 2010. View at Publisher · View at Google Scholar · View at Scopus
  36. C. Iadecola, L. Park, and C. Capone, “Threats to the mind: aging, amyloid, and hypertension,” Stroke, vol. 40, no. 3, supplement, pp. S40–S44, 2009. View at Publisher · View at Google Scholar · View at Scopus
  37. R. Kovács, S. Schuchmann, S. Gabriel, O. Kann, J. Kardos, and U. Heinemann, “Free radical-mediated cell damage after experimental status epilepticus in hippocampal slice cultures,” Journal of Neurophysiology, vol. 88, no. 6, pp. 2909–2918, 2002. View at Google Scholar · View at Scopus