Amelogenesis Imperfecta and Screening of Mutation in Amelogenin Gene

Table 1

Primers sequence (forward and reverse) of exons of the amelogenin gene and the PCR conditions.

Exon	Primer sequence 5′-3′	Fragment size (bp)	AT (°C)
2	F: AGATTATGTGTGTTTTATGGAGCA	233	62°C
	R: CCCTAATTTCACCAACTATGAGC
3	F: TCCTTTAATGTGAACAATTGCAT	250	57°C
	R: TCTGGGATAAAGAATCAACACA
4-5	F: AATGAATCTCTTTAACTCCCCATAA	367	60°C
	R: TCCCATTAATGTCTGCATGTG
6	F: CCATAATGGCAAAGAAAACAC	599	59°C
	R: TGGTTGTCGGAGACCTTAGAA
7	F: TGACAAAACTGAAGCCAGACAT	237	61°C
	R: TGCATTTTATTGTCTGCTAATGG

F: forward; R: reverse; bp: base pairs; AT: annealing temperature.