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Case Reports in Genetics
Volume 2014, Article ID 906145, 2 pages
http://dx.doi.org/10.1155/2014/906145
Case Report

First Birth after Sperm Selection through Discontinuous Gradient Centrifugation and Artificial Insemination from a Chromosomal Translocation Carrier

1Medical Genetics and Embryology Department, AP-HP, Trousseau Hospital, 28 avenue du Docteur Arnold Netter, 75012 Paris, France
2Reproductive Medicine Department, Bluets Hospital, 4 rue Lasson, 75012 Paris, France
3Drouot Laboratory, 21 rue Drouot, 75009 Paris, France

Received 3 October 2013; Accepted 17 November 2013; Published 23 January 2014

Academic Editors: D. Steinberger and C. Yapijakis

Copyright © 2014 Alexandre Rouen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. Balanced chromosomal carriers, though usually healthy, are confronted with recurrent spontaneous abortions and malformations in the offspring. Those are related to the transmission of an abnormal, chromosomally unbalanced genotype. We evidenced that the proportion of unbalanced spermatozoa can be significantly decreased through a sperm preparation process called discontinuous gradient centrifugation (DGC). We therefore started offering intrauterine inseminations with this procedure to couples with a male translocation carriers. Case Presentation. We report the case of a 37-year-old man carrying a t(3;10)(q25;p13) reciprocal translocation. He and his partner had had trouble conceiving for ten years and had four spontaneous abortions. DGC in this patient decreased the proportion of unbalanced spermatozoa from 63.6% to 52.3%. They were therefore offered intrauterine insemination with DGC, which eventually led to the birth of a healthy female child carrying the paternal translocation. Conclusion. We showed that translocation carriers could be offered intrauterine inseminations with DGC. Before this, the only two options were natural conception with prenatal diagnosis and termination of chromosomally unbalanced fetuses or preimplantation genetic diagnosis, which is a much heavier and costly procedure. We are currently offering this option through a multicentric program in France, and this is the first birth originating from it.