<i>FOXE1</i> Mutation Screening in a Case with Cleft Lip, Hypothyroidism, and Thyroid Carcinoma: A New Syndrome?

Mendieta-Zerón, Hugo; Jiménez-Rosales, Angélica; Pérez-Amado, Carlos Jhovani; Jiménez-Morales, Silvia

doi:https://doi.org/10.1155/2017/6390545

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Case Report | Open Access

Volume 2017 | Article ID 6390545 | https://doi.org/10.1155/2017/6390545

FOXE1 Mutation Screening in a Case with Cleft Lip, Hypothyroidism, and Thyroid Carcinoma: A New Syndrome?

Hugo Mendieta-Zerón,^1,2Angélica Jiménez-Rosales,¹Carlos Jhovani Pérez-Amado,³and Silvia Jiménez-Morales⁴

Academic Editor: Balraj Mittal

Received03 May 2017

Revised19 Jul 2017

Accepted30 Jul 2017

Published27 Aug 2017

Abstract

A 26-year-old woman is referred to the Internal Medicine consultation due to increases in laboratory studies associated with Papillary Thyroid Carcinoma (PTC) that was confirmed by histopathological studies. Her clinical history revealed that, at 3 months of age, she was successfully treated with surgery for cleft lip (CL) and at the age of 24 years was diagnosed with hypothyroidism. Single nucleotide polymorphisms (SNPs) in FOXE1 and its promoter regions have been associated with various etiologies related to the thyroid, including orofacial clefting, specially cleft palate (CP) and CL, hypothyroidism (HT), and thyroid cancer. The association of CL, HT, and PTC might be component of a new syndrome; however FOXE1 coding region, which has been involved with these entities, has not exhibited mutations or SNPs. Further study of other genes may help in better characterization of the possible syndrome.

1. Introduction

Transcription factors NKX2-1, FOXE1, HHEX, and PAX8 are involved in cellular differentiation during embryogenesis. They play a critical role in the morphogenesis, differentiation, and maintenance of the thyroid gland. The FOXE1 gene encodes for a transcription factor protein that is expressed from the embryonic stage in the thyroid primordium until the development of the thyroid gland by the regulation of thyroid promoters [1]. Single nucleotide polymorphisms (SNPs) in this gene and its promoter regions have been associated with various etiologies related to the thyroid, including orofacial clefting, especially cleft palate (CP) and cleft lip (CL), hypothyroidism (HT), and thyroid cancer (TC).

CP and CL are common birth defects, particularly in Asian and Native American population, which have the highest rates of prevalence, in contrast to African population, which has the lowest. Both have a complex etiology, in that they possess multigenetic and multifactorial causes that have not been elucidated. However, research and mapping of the 9q22-q33 guided the associations with several SNPs located in the FOXE1 locus [2]. Actually, the genotypes involving the commonest alleles of rs3758249 (GG) and rs4460498 (CC) were the most associated with CP in Caucasian and Asian derived populations [3–5]. To note, both SNPs are located in a high linkage disequilibrium (LD) region, which also harbors the rs1867277 (−283G>A FOXE1) SNPs that have been reported as functional in thyroid cancer [6].

HT is the most common thyroid disorder. It is characterized by low production of thyroid hormones T3 and T4. Through Genome-Wide Association Studies (GWAS), various polymorphisms, including SNP located in the FOXE1 gene, have been identified with problems in the formation and differentiation of the thyroid, producing a predisposition to the disease [5].

TC is the most common endocrine malignancy, with a strong genetic component that has been shown to extend beyond the nuclear family. Histologically, it is classified as Papillary (PTC), Follicular (FTC), and Medullary (MTC) Carcinomas, and undifferentiated anaplastic thyroid carcinomas, and studies have suggested that gender, age, tumor size, histologic type, tumor infiltration, and vascular/lymphatic invasion affect clinical outcome and treatment options [13]. Evidence in multiple ethnicities has associated SNP with the same LD region as FOXE1 with PTC and MTC [1, 6]. The case described here represents unexpected combination of CL, HT, and PTC.

2. Case Report

A 26-year-old woman from Jalisco (Mexico) is referred to the Internal Medicine consultation due to increases in laboratory studies associated with PTC. Her clinical history revealed that, at 3 months of age, she was successfully treated with surgery for CL, at 14 years of age underwent a cholecystectomy, and at the age of 24 years was diagnosed with HT. In April 2016, her clinical examinations revealed that she had anti-thyroglobulin antibodies (>4,000 μg/L) and anti-peroxidase antibodies > 42 IU/mL (normal reference value, 34). Imaging studies demonstrated multinodularity (Ultrasound [US]) (Figure 1(a)), discrete thyroid growth (Figure 1(b)) and the thyroid fine needle aspiration (FNA) biopsy corroborated PTC Bethesda Class V (Figure 2).

(a)

(b)

Based on the contribution of FOXE1 mutations and SNPs variants (Figure 3) in the development of the CL, HT, and PC, we sequenced the exon 1 of this gene (coding region and 3′UTR) in order to identify mutations or SNPs potentially associated with the patient’s diseases. At the Research Laboratory of Ciprés Grupo Médico S.C. (CGM), Genomic DNA was isolated from peripheral white blood cells using the DNA purification kit (Quick-DNA Miniprep Plus Kit, Zymo Research Corp) according to the manufacturer’s instructions. At the INMEGEN Exon 1 of FOXE1 gene was analysed using the primers described in Table 1. PCR was performed in a final volume of 50 μl, using 50 ng of genomic DNA, 2.5 pmoles of each primer, 2 mM of each dNTP, 1 U of AmpliTaq Gold DNA Polymerase, 4 mM MgCl₂, and 10x PCR Gold buffer (Applied Biosystems provided polymerase, MgCl₂, and buffer). Cycling conditions for PCR consisted of a first denaturation step of 95°C for 10 min, followed by 35 cycles of denaturation at 95°C for 20 s, annealing at 60°C for 20 s, and extension at 72°C for 30 s, followed by a final extension at 72°C for 7 min. The runs were carried out on a GeneAmp PCR system 9700 (Applied Biosystems). PCR products were sequenced directly with a DNA Sequencing Kit with Big Dye Terminator on an automated ABI 3730XL sequencer (Applied Biosystems, Foster City, CA, USA) and data analyses were performed by Lasergene software (DNASTAR Inc.). Neither mutations nor SNPs were found in FOXE1 coding region. Two SNPs (rs1867279 and rs1867280) that have already been associated with CP were found in a homozygote wild type genotype. Additionally, we found the rs71369530 (Ala-14) in homozygote fashion. Functional prediction analysis using the SNPinfo Web Server (https://snpinfo.niehs.nih.gov/snpinfo/snpfunc.html) showed that only the ancestral alleles of rs1867279 and rs1867280 might recruit the E2F and NFKB transcription factors, respectively.

3. Discussion

3.1. Generalities

Several studies in genetics have shown that HT, TC, and isolated CL and CP are related to the FOXE1 locus. FOXE1 (Forkhead box E1; UniGene accession number Hs.159234) is an intron-less single exon gene that encodes transcription factor FOXE1 (or TTF-2). FOXE1 is an 373 amino acid protein (38 kDa) that contains a forkhead winged helix DNA binding domain and a polyalanine (polyAla) tract of variable length (11–19 Ala, but 14-Ala is most abundant) and negatively regulates thyroglobulin (TG) and Thyroid Peroxidase (TPO) expression, which regulates, in conjunction with NKX2-1 (Homeobox protein or TTF-1), PAX8, and HHEX, thyroid differentiation and function [9, 17].

RNA in situ hybridization studies in zebrafish in the fish ortholog of FOXE1, foxe1, indicated that during the postfertilization development, the gene is early expressed in the nervous system, afterward in the oral epithelium and thyroid gland, later in the developing heart, and, in the final stages, in the pharyngeal arch epithelium [5]. Moreover, phenome-wide association studies of genotypes of electronic medical records in DNA biobanks have associated the FOXE1 locus with thyroiditis, nodular and multinodular goiters, and thyrotoxicosis, among others [18], indicating that mutations in this gene and its promoters exert an important effect on normal thyroid function.

3.2. Orofacial Clefts

Orofacial clefts (OC) comprise clefts, disruption, or gaps in structures such as lips, palate, eyes, and nose and arise from failure of normal craniofacial development, in which cells grow, migrate, and differentiate in a coordinated fashion to fuse together the lateral, medial, and maxillary processes. Moreover, given the different developmental origins of lip and palate, these are classified in CL with or without CP [19].

Isolated CL and CP are common genetic defects with a strong genetic component that has not been completely elucidated, and approximately 70–80% of CL/CP that are nonsyndromic (absence of additional structural or cognitive abnormalities) represent complex human disorders with the interaction of genetic risk factors and environmental exposures that increase susceptibility [19]. Evidence suggests that rare DNA variations in several genes and SNPs (many of these are present in noncoding DNA) are linked to CL and CP etiology [3, 8].

3.3. Relation of FOXE1 with Orofacial Clefts

Genome-wide association studies (GWAS) have related FOXE1 with CL and CP in different population, within the region 9q22-33 [3, 5, 8]; however the SNP and haplotype frequencies found within each population indicate multiple risk alleles for such a complex disease, due in part to maternal or paternal overtransmission [3]. Moreover, as its role with the expression of TG and TPO suggests, mutations at the FOXE1 locus in 9q22 have been associated with thyroid disorders such as congenital HT due to thyroid dysgenesis, primary HT, goiters, nonmedullary thyroid cancer, PTC, and TC [5, 8].

FOXE1 is responsible for palatogenesis, and it appears that casual mutations in noncoding regions that regulate FOXE1 expression are related to CL and CP [3], such as SNPs rs7850258, rs12342417, and rs10984103 within the FOXE1 locus that have been associated with high risk for orofacial clefting [8].

Few studies have been carried out on Mexican population or in persons with American-Indian ethnicity; however, research has shown that SNP rs4460498 and rs375829, located in the FOXE1 locus, are associated with nonsyndromic CP and CL for European and Mayan-Mesoamerican descendants [7]. This study obtained important significance in high risk of CL/CP for rs4460498 = 6.50 × 10⁻⁶ and = 0.051, and for rs375829 = 2.41 × 10⁻⁵ and = 0.0299.

3.4. Relation of FOXE1 with Hypothyroidism

HT is the most common thyroid disorder characterized by deficiencies of thyroid hormones T3 and T4 (triiodothyronine and thyroxine, resp.), which are regulators of metabolism and development. A common marker of HT is the high levels of Thyroid-Stimulating Hormone (TSH), which indicate impaired thyroid function; however, in other cases, reduced levels of TSH cause low levels of T3 and T4. Iodine deficiency is the most common cause of HT, but congenital HT and autoimmune HT are other varieties with different genetic causes [20] that cause central HT, primary HT, alterations in thyroid transcription factors (with which the FOXE1 gene is associated), dyshormonogenesis, and other genes such as the TG and TSH genes [19].

A mutation in the coding sequence of FOXE1 that shifts Arginine-102 to a Cysteine (R102C), a highly conserved residue within the forkhead domain of the protein, impairs FOXE1 from binding DNA; thus, it is inactive toward transcription. This inactivity leads to thyroid dysgenesis in the patient, which causes congenital HT, CP, spikey hair, and bilateral choanal atresia. Interestingly, because athyreosis is not present, human thyroid formation and development do not depend entirely on FOXE1 activity [21].

SNPs that are present in other autoimmune diseases, such as rs6679677 near PTPN22, rs3184504 in SH2B3, and rs2517532 in HLA class I region, are linked to HT, while rs4915077 near VAV3 [20] and other SNPs such as rs7850258, rs965513, rs925489, and rs10759944, all located near FOXE1 in chromosome 9, exhibit genome-wide significance with primary HT [18]. Other SNPs in chromosome 9 that appear to be associated with primary HT are rs4979402, rs4979397, rs1408528, and rs1535971, located in DFNB31 that is 16.6 Mb from FOXE1 and not in LD with other SNP of FOXE1; however, these later regions do not correlate when TSH is not expressed [18].

3.5. Relation of FOXE1 with Thyroid Cancer

TC is a complex polygenic disorder and the networking of the molecular mechanisms involved in its development, the genes involved in the process, and the risk environmental factors remain to be understood. It has been proposed that TC is the result of multiple low-to moderate penetrance genes interacting with each other, acting together with other transcriptional regulators that produce loss- or gain-of-function and that, together with environmental and other genetic risk factors, can lead to the development of cancer [6, 14, 21]. However, this explains only a small portion of the incidence, as there are other genetic factors involved. TC types, such as FTC, Differentiated Thyroid Cancer (DTC, which includes PTC and MTC), and undifferentiated anaplastic thyroid carcinomas, have different biomarkers and cellular conformations and thus different molecular pathways involved in their development.

rs7850258, located in the FOXE1 promoter region near craniofacial enhancer hsCNE-67, has direct involvement in the development of thyroid and heart, and is also associated with HT and TC, as it was found to alter the enhancer function in both oral epithelial and thyroid cell lines. In this SNP, allele G is associated with HT and CL, whereas allele A is associated with TC [5].

Studies in several countries [6, 9–11, 14–16] have revealed that FOXE1 locus polymorphisms in chromosome 9q22.33, such as rs965513 and polymorphisms (rs7849497, rs1867277, rs1867278, rs1867279, and rs1867280) in the promoter regions for this factor are associated with an increased risk of nonmedullary thyroid cancer, differentiated thyroid cancer, and the Bamforth–Lazarus syndrome, in which the mutation of a serine-57 to asparagine (S57N) in the forkhead-DNA binding domain of FOXE1 leads to CP, choanal atresia, bifid epiglottis, thyroid agenesis or dysgenesis, hypothyroidism, and spikey hair [22]. Although there is no clear evidence of the role of the rs1867279 and rs1867280 in CL, HT, and PTC pathogenesis, functional prediction analysis showed that both SNPs could modify the transcription factor binding site and thus modulate the expression of relevant genes in the thyroid morphogenesis. Otherwise, the rs1867279 and rs1867280 might be in LD with the true casual variant, such as rs1867277, because a high LD has been described in the FOXE1 region. On this regard, it has been reported that the rs1867277A allele recruits the USF1/USF2 transcription factors and has been shown to be involved in an allele-specific transcriptional regulation of FOXE1 in thyroid cancer [6]. Actually, the rs1867277 have been found related to TC in several populations [21, 23, 24].

The information about the association of the FOXE1 SNP rs1867277 with TC appears to vary with ethnicity and cancer type [16, 23]. For example Bychkov et al. [12] found overexpression of rs1867277 and rs965513 by immunohistochemical staining in PTC in Japanese persons. On the contrary, neither Maillard et al. [14] in French Polynesians nor Bonora et al. [25] in European descendants found an association of this SNP with nonmedullary thyroid cancer.

SNP rs1867277, within the −283G>A promoter region, was assessed functionally by Landa et al. [6] and their results suggest that this SNP increases the transcriptional activity of the FOXE1 gene promoter by recruitment of leucine zipper USF 1 and 2. Thus, it is likely that recruiting USF1 and USF2 proteins produces a deregulation of FOXE1 that leads to malignant behavior of thyroid cells [9].

It is been demonstrated that, through a number of signalling pathways, FOXE1, along with the remainder of the family of FOX transcription factors, can induce WNT5A, a gene (located in 3p14.3) expressed in a variety of human tumors [26]. Thus, by deregulation of FOXE1, it is likely that this protein can become a tumor suppressor or an oncogene through the Wnt pathway, either dependently or independently of β-catenin [17]. Also, there is evidence that the RET/PTC3 protooncogene that causes TC decreases FOXE1 expression. Similarly, in squamous cell carcinoma, the 9q22 region of the chromosome (where FOXE1 is localized) is lost and, in other cancers, hypermethylation of promoter is also observed [5].

Levels of FOXE1 and PAX8 messenger RNA (mRNA) in thyroid nodules have been found to be decreased in malignant thyroid lesions and higher in benign lesions, and even the gene expression patterns of FOXE1 and PAX8 can help in differentiating FTC from MTC and the Follicular Variant of Papillary Thyroid Carcinoma (FVPTC) [27].

Because we did not sequence the entire gene, our analysis did not exclude the hypothesis that FOXE1 mutations contribute to the development of these diseases. Numerous evidences have been published suggesting that this gene is involved in these three diseases. In the same haplotype analysis of the members of a Portuguese family with nonmedullary thyroid carcinoma, the selected individuals with the disease not only shared the FOXE1 p.A248G variant, but also the same haplotype between markers rs965512 and D99S180 (located in chromosome 9), including rs1867279 and rs1867280 (SNPs present in our patient). However, these persons did not exhibit an association with any of the other polymorphic loci with the cancer because, as according to the authors and as reported in a previous paper, 15% of Portuguese control population have these SNP [17]. However, we propose that ethnicity and environmental factors are very important in triggering a disruptive response in rs1867279 and rs1867280, as in the case of our patient, in whom these SNPs are acting together, along with other risk factors (ethnic and environmental), to produce CL, HT, and TC.

Given the puzzling findings reported with rs1867277 [12, 16, 22] in FOXE1 or rs944289 in NKX2 [15], it is obvious that ethnicity and environmental factors play a key role for the development of TC and other malignancies. Additionally, the involvement of FOXE1 in thyroid formation and function, as well as the formation of craniofacial structures, relates mutations in the noncoding region of FOXE1 to the phenotype of our patient.

Evidence such as the Bamforth–Lazarus syndrome [22], or mutations in FOXE1 that explain an association of hypothyroidism and cleft palate [28], in addition to observational and comparative data of human and animal models null for Foxe1 [5, 29] that are consistent with CP, CL, and other thyroid abnormalities, suggests that mutations or SNP in FOXE1 can be a common cause for a possible new syndrome characterized by CL, HT, and PTC. In this line, Tables 2 and 3 illustrate some of the most studied SNPs and genes probably associated with these diseases.

Seeking an explanation for the possible identification of the syndrome that has been discussed, the FOXE1 mutation has been tested, being negative as far as could be analysed. A possible candidate gene in this situation is E-cadherin CDH1 on 16q22.1, which is also expressed in critical stages of lip and palate development in embryos [30, 31]. Hence, there are many more genetic alterations that could explain this possible association.

4. Conclusions

The association of CL, HT, and PTC might be component of a new syndrome; however FOXE1 coding region, which has been involved with these entities, has not exhibited mutations or SNPs. Based on this case study and a review of the literature on the role that the FOXE1 gene plays in these pathologies, a more extensive sequence analysis (including other genes) is needed to rule out its role in the above-mentioned diseases.

Ethical Approval

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000.

Informed consent was obtained from all patients for being included in the study.

Conflicts of Interest

All authors declare that there are no conflicts of interest.

Authors’ Contributions

All authors made substantial contributions to the conception or design of the work, or the acquisition, analysis, or interpretation of data for the work, drafting the work, or revising it critically for important intellectual content, gave final approval of the version to be published, and agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Acknowledgments

Funding was provided by Ciprés Grupo Médico S.C. (CGM) and by the Instituto Nacional de Medicina Genómica (INMEGEN).

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Copyright © 2017 Hugo Mendieta-Zerón et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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