Case of Inherited Partial AZFa Deletion without Impact on Male Fertility

Table 1

Markers, used for the detection of AZFa region deletion size (#Wu et al. [12], MSY Breakpoint Mapper [13]), F — forward, R — reverse.

STS	Genomic coordinates (GRCh38)	Primer	Size of expected PCR product
sy85	Y:12525881–12526250	F — TGGCAATTTGCCTATGAAGT	369 bp
		R — ACAGGCTATTTGACTGGCAG
sY84#	Y:12678105–12678432	F — GCTGAGGAGTTGTGGAGACC	642 bp
		R — GCAAGGACATTCCAGGGTTA
sY1323 (intragenic)	Y:12721978–12722325	F — ATGGTGAATATAATATAGGCAGAATTT	348 bp
		R — CCTTACCAGGAAGGTTTGTGA
USP9Y-44 (intragenic)	Y:12856484–12856970	F — CCAGATCTTACAGGTGAGGGTTT	487 bp
		R — GCAAACAAAACTGCACATGATT
sY87	Y:12897218–12897468	F – TCTGTTGCTTGAAAAGAGGG	251 bp
		R — ACTGCAGGAAGAATCAGCTG
sY1234 (intragenic)	Y:12914758–12915107	F — TTACCCCTTTCACCCACTGA	350 bp
		R – CCATAAACTACACAAGGACGAACT
DDX3Y-16 (intragenic)	Y:12917260-12917716	F — TGGGACATTAATGGGATGGT	457 bp
		R – GTTGCCACCCACCTGTAATC