Case Reports in Hematology

Case Reports in Hematology / 2017 / Article

Case Report | Open Access

Volume 2017 |Article ID 7657393 |

Stephen E. Langabeer, Lisa Preston, Johanna Kelly, Matt Goodyer, Ezzat Elhassadi, Amjad Hayat, "Molecular Profiling: A Case of ZBTB16-RARA Acute Promyelocytic Leukemia", Case Reports in Hematology, vol. 2017, Article ID 7657393, 4 pages, 2017.

Molecular Profiling: A Case of ZBTB16-RARA Acute Promyelocytic Leukemia

Academic Editor: Massimo Gentile
Received24 Feb 2017
Accepted09 Apr 2017
Published26 Apr 2017


Several variant RARA translocations have been reported in acute promyelocytic leukemia (APL) of which the t(11;17)(q23;q21), which results in a ZBTB16-RARA fusion, is the most widely identified and is largely resistant to therapy with all-trans retinoic acid (ATRA). The clinical course together with the cytogenetic and molecular characterization of a case of ATRA-unresponsive ZBTB16-RARA APL is described. Additional mutations potentially cooperating with the translocation fusion product in leukemogenesis have been hitherto unreported in ZBTB16-RARA APL and were sought by application of a next-generation sequencing approach to detect those recurrently found in myeloid malignancies. This technique identified a solitary, low level mutation in the CEBPA gene. Molecular profiling of additional mutations may provide a platform to individualise therapeutic management in patients with this rare form of APL.

1. Introduction

Acute promyelocytic leukemia (APL) is a distinct form of acute myeloid leukemia (AML) characterized by the balanced translocation t(15;17)(q24;q21) that results in production of the PML-RARA oncogene. Retinoid-based therapy in combination with either anthracyclines or arsenic trioxide results in favorable rates of complete remission and overall survival, provided that the patient can be supportively managed through the initial coagulopathy [1]. However, a small proportion of patients harbor variant translocations that result in fusion of RARA to one of a number of alternative partner genes [2, 3]. The most often reported of these variant translocations is the t(11;17)(q23;q21) which results in the fusion of the zinc finger gene ZBTB16 (formerly PLZF) to the RARA locus [4, 5]. The bone marrow morphology of patients with ZBTB16-RARA APL tends to be distinct from those patients with either classical or the hypogranular variant of APL [6]. Identification of the ZBTB16-RARA fusion is critical for therapeutic purposes as these patients are generally resistant to differentiating retinoid therapy, specifically all-trans retinoic acid (ATRA) [7], and treatment should be with standard AML regimens according to current recommendations [8].

In murine models of APL, induction of PML-RARA expression in myeloid stem cells results in a myeloproliferative disease that subsequently develops into leukemia with promyelocytic features after a relatively long latency implicating the requirement for further cooperating mutations to fully recapitulate the APL phenotype [9, 10]. The development and application of whole exome sequencing and targeted exome sequencing have led to the identification of several cooperating mutations in APL and demonstrates the clonal and subclonal acquisition of mutational events in conjunction with the driver PML-RARA oncogene [11, 12]. A similar pattern of these cooperative mutations appears to exist within patients with APL and those with other types of AML [13]. Whether these additional mutations have a prognostic impact is unclear [14]. However, identification of these mutations may allow targeted intervention [15]. To date, the pattern of cooperating mutations in patients with ZBTB16-RARA APL has not been investigated. Characterization of a patient with this uncommon cytogenetic variant of APL is described with subsequent application of a targeted next-generation sequencing (NGS) approach to identify allied mutational events.

2. Case Report

An 81-year-old female presented with a short history of back pain with a physical examination proving unremarkable. Full blood count revealed pancytopenia (hemoglobin 8.8 g/dl, neutrophils 1.8 109/L, and platelets 140 109/L) and abnormal circulating promyelocytes that constituted 40% of nucleated cells. A coagulation screen was only mildly deranged with a prothrombin time of 15.1 seconds, an activated partial thromboplastin time of 29.4 seconds, and fibrinogen of 3.2 g/L. Peripheral blood promyelocytes, which accounted for 40% of nucleated cells, had regular nuclei, were hypergranular, and lacked Auer rods (Figure 1(a)) with increased numbers of pseudo-Pelger-Huet neutrophils noted (Figure 1(b)). Immunophenotyping detected autofluorescent blasts that expressed MPO, CD13, CD14, and CD33 but lacked CD34 and HLA-DR expression. The bone marrow was effaced by promyelocytes (Figure 1(c)) which stained strongly positive for Sudan Black. The patient commenced ATRA with a progressive concurrent increase noted in both peripheral blood myeloblasts and promyelocytes (white cell count 40.5 109/L), thrombocytopenia (platelets 92 109/L), and coagulopathy (fibrinogen 0.7 g/L) despite intensive blood product support. The chest findings were more consistent with aspiration and therefore no response to ATRA with differentiation syndrome excluded. Hydroxyurea and idarubicin were initiated in an attempt to achieve cytoreduction; however, progressive respiratory compromise and worsening coagulopathy led to the patients’ death due to pulmonary hemorrhage ten days after presentation.

Cytogenetic G-band analysis identified an aberration involving 17q in 4/13 metaphases analysed. Metaphase fluorescent in situ hybridisation studies revealed a rearrangement involving RARA at 17q12 and confirmed the partner chromosome to be 11q, thus identifying the t(11;17) translocation with a full karyotype of 46,XX,add(17)(q21)[]/46,XX[].ish der(11)t(11;17)(q23;q21)(RARA+).nuc ish(PMLx2,RARAx3)[142/200]. A standardised reverse transcription-PCR approach [16] did not detect PML-RARA transcripts, but, using primers previously described [7], a ZBTB16-RARA fusion transcript, consistent with fusion of ZBTB16 exon 3 fused to RARA exon 2, was detected and confirmed by Sanger sequencing (Figure 2). The reciprocal RARA-ZBTB16 fusion was not detected.

An NGS approach utilising a gene panel to detect additional mutations cooperating with the ZBTB16-RARA fusion in propagating APL was retrospectively employed. Amplicon libraries covering thirty commonly mutated genes implicated in myeloid malignancies were generated using genomic DNA from the diagnostic bone marrow aspirate using an Ion AmpliSeq™ approach (Thermo Fisher Scientific, Paisley, UK). A single in-frame CEBPA p.P189delP mutation was detected (c.564–566 delGCC; reference sequence GRCh37: 19:33792755-7) with an allele frequency of 6.2%.

3. Discussion

Several rare variant RARA translocations have been described in APL among which the ZBTB16-RARA is the most frequent. Identification of the t(11;17) at the cytogenetic and/or the ZBTB16-RARA fusion at the cytogenetic and molecular levels, respectively, is paramount as, in the case described herein, ZBTB16-RARA APL is generally unresponsive to ATRA therapy, although it must be noted that some response to initial ATRA therapy has been documented in very rare cases and always in combination with other agents [1719]. Although distinctive morphological features have been ascribed to ZBTB16-RARA APL [6], the peripheral blood and bone marrow morphology in this case more closely resembled that of typical APL, an aspect infrequently reported [20].

The NGS approach adopted herein detected only one additional mutation in CEBPA which encodes a transcription factor involved in cell fate decisions for myeloid cell differentiation [21]. This gene is recurrently mutated in types of AML other than APL, but mutations at this codon have been rarely documented [22]. Although no constitutional material was available and therefore not analysed in tandem, the low allele frequency implies that this is an acquired, somatic mutation of CEBPA and not a single nucleotide polymorphism. How CEBPA mutations might cooperate with the ZBTB16-RARA fusion in leukemogenesis is unclear; however, one study has demonstrated that CEBPA protein activity is severely impaired in leukemic promyelocytes with the t(11;17) translocation and that the reciprocal RARA-ZBTB16 protein inhibits myeloid cell differentiation through its interaction with CEBPA [23]. It is acknowledged that this NGS approach is limited to those commonly mutated genes in myeloid malignancies and that a greater coverage of both other genes and additional exons of those genes within this panel may prove more informative.

In conclusion, rapid identification and comprehensive molecular profiling are vital for future appropriate tailoring of therapy in patients with this rare form of APL.


Current address of Matt Goodyer is Service d’Hématologie, Hôpital du Valais, 1950 Sion, Switzerland, and current address of Ezzat Elhassadi is Department of Haematology, University Hospital Waterford, Waterford, Ireland.

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.


The authors are grateful to Kathleen Brosnan, Cancer Molecular Diagnostics, St. James’s Hospital, Dublin, Ireland, for technical assistance with next-generation sequencing.


  1. L. Cicconi and F. Lo-Coco, “Current management of newly diagnosed acute promyelocytic leukemia,” Annals of Oncology, vol. 27, no. 8, pp. 1474–1481, 2016. View at: Publisher Site | Google Scholar
  2. J. Adams and M. Nassiri, “Acute promyelocytic leukemia a review and discussion of variant translocations,” Archives of Pathology and Laboratory Medicine, vol. 139, no. 10, pp. 1308–1313, 2015. View at: Publisher Site | Google Scholar
  3. W. Yan and G. Zhang, “Molecular characteristics and clinical significance of 12 fusion genes in acute promyelocytic leukemia: a systematic review,” Acta Haematologica, vol. 136, no. 1, pp. 1–15, 2016. View at: Publisher Site | Google Scholar
  4. S.-J. Chen, A. Zelent, J.-H. Tong et al., “Rearrangements of the retinoic acid receptor alpha and promyelocytic leukemia zinc finger genes resulting from t(11;17)(q23;q21) in a patient with acute promyelocytic leukemia,” Journal of Clinical Investigation, vol. 91, no. 5, pp. 2260–2267, 1993. View at: Publisher Site | Google Scholar
  5. J. D. Licht, C. Chomienne, A. Goy et al., “Clinical and molecular characterization of a rare syndrome of acute promyelocytic leukemia associated with translocation (11;17),” Blood, vol. 85, no. 4, pp. 1083–1094, 1995. View at: Google Scholar
  6. D. Sainty, V. Liso, A. Cantù-Rajnoldi, and et al., “A new morphological classification system for acute promyelocytic leukemia distinguishes cases with underlying PLZF/RARA gene rearrangements,” Blood, vol. 96, no. 4, pp. 1287–1296, 2000. View at: Google Scholar
  7. F. Guidez, W. Huang, J. H. Tong et al., “Poor response to all-trans retinoic acid therapy in a t(11;17) PLZF/RAR alpha patient,” Leukemia, vol. 8, no. 2, pp. 312–317, 1994. View at: Google Scholar
  8. M. A. Sanz, D. Grimwade, M. S. Tallman et al., “Management of acute promyelocytic leukemia: recommendations from an expert panel on behalf of the European LeukemiaNet,” Blood, vol. 113, no. 9, pp. 1875–1891, 2009. View at: Publisher Site | Google Scholar
  9. L. M. Kelly, J. L. Kutok, I. R. Williams et al., “PML/RARα and FLT3-ITD induce an APL-like disease in a mouse model,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 12, pp. 8283–8288, 2002. View at: Publisher Site | Google Scholar
  10. L. D. Wartman, D. E. Larson, Z. Xiang et al., “Sequencing a mouse acute promyelocytic leukemia genome reveals genetic events relevant for disease progression,” Journal of Clinical Investigation, vol. 121, no. 4, pp. 1445–1455, 2011. View at: Publisher Site | Google Scholar
  11. J. S. Welch, T. J. Ley, D. C. Link et al., “The origin and evolution of mutations in acute myeloid leukemia,” Cell, vol. 150, no. 2, pp. 264–278, 2012. View at: Publisher Site | Google Scholar
  12. V. Madan, P. Shyamsunder, and L. Han, “Comprehensive mutational analysis of primary and relapse acute promyelocytic leukemia,” Leukemia, vol. 30, no. 8, pp. 1672–1681, 2016. View at: Google Scholar
  13. L. Riva, C. Ronchini, M. Bodini et al., “Acute promyelocytic leukemias share cooperative mutations with other myeloid-leukemia subgroups,” Blood Cancer Journal, vol. 3, no. 9, p. e147, 2013. View at: Publisher Site | Google Scholar
  14. R. Dillon and D. Grimwade, “Prognostic significance of additional cytogenetic abnormalities and FLT3 mutations in acute promyelocytic leukemia,” Leukemia & Lymphoma, vol. 55, no. 7, pp. 1444–1446, 2014. View at: Publisher Site | Google Scholar
  15. C. Lai, J. E. Karp, and C. S. Hourigan, “Precision medicine for acute myeloid leukemia,” Expert Review of Hematology, vol. 9, no. 1, pp. 1–3, 2016. View at: Publisher Site | Google Scholar
  16. J. J. M. Van Dongen, E. A. Macintyre, J. A. Gabert et al., “Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. Report of the BIOMED-1 concerted action: investigation of minimal residual disease in acute leukemia,” Leukemia, vol. 13, no. 12, pp. 1901–1928, 1999. View at: Publisher Site | Google Scholar
  17. D. J. Culligan, D. Stevenson, Y.-L. Chee, and D. Grimwade, “Acute promyelocytic leukaemia with t(11;17)(q23;q12-21) and a good initial response to prolonged ATRA and combination chemotherapy,” British Journal of Haematology, vol. 100, no. 2, pp. 328–330, 1998. View at: Publisher Site | Google Scholar
  18. J. H. Jansen, M. C. de Ridder, W. M. C. Geertsma et al., “Complete remission of t(11;17) positive acute promyelocytic leukemia induced by all-trans retinoic acid and granulocyte colony-stimulating factor,” Blood, vol. 94, no. 1, pp. 39–45, 1999. View at: Google Scholar
  19. M. C. Petti, F. Fazi, M. Gentile et al., “Complete remission through blast cell differentiation in PLZF/RARα-positive acute promyelocytic leukemia: In vitro and in vivo studies,” Blood, vol. 100, no. 3, pp. 1065–1067, 2002. View at: Publisher Site | Google Scholar
  20. S. B. Han, J. Lim, Y. Kim, H. Kim, and K. Han, “A variant acute promyelocytic leukemia with t(11;17)(q23;q12); ZBTB16-RARA showing typical morphology of classical acute promyelocytic leukemia,” The Korean Journal of Hematology, vol. 45, no. 2, pp. 133–135, 2010. View at: Publisher Site | Google Scholar
  21. E. Ohlsson, M. B. Schuster, M. Hasemann, and B. T. Porse, “The multifaceted functions of C/EBPα in normal and malignant haematopoiesis,” Leukemia, vol. 30, no. 4, pp. 767–775, 2016. View at: Publisher Site | Google Scholar
  22. L.-I. Lin, C.-Y. Chen, D.-T. Lin et al., “Characterization of CEBPA mutations in acute myeloid leukemia: Most patients with CEBPA mutations have biallelic mutations and show a distinct immunophenotype of the leukemic cells,” Clinical Cancer Research, vol. 11, no. 4, pp. 1372–1379, 2005. View at: Publisher Site | Google Scholar
  23. N. Girard, M. Tremblay, M. Humbert et al., “RARα-PLZF oncogene inhibits C/EBPα function in myeloid cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 33, pp. 13522–13527, 2013. View at: Publisher Site | Google Scholar

Copyright © 2017 Stephen E. Langabeer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

More related articles

 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles