Splenic B-Cell Lymphomas with Diffuse Cyclin D1 Protein Expression and Increased Prolymphocytic Cells: A Previously Unrecognized Diagnostic Pitfall

Figure 5

Case 2: peripheral blood and bone marrow biopsy. Wright Giemsa-stained peripheral blood smear shows lymphocytosis with many of the circulating lymphoma cells being small in size with round nuclear contours and minimal cytoplasm (a) 500x magnification. Approximately 50% of circulating lymphoma cells had more abundant cytoplasm and prominent nucleoli, consistent with prolymphocytic morphologic features (b) 1000x magnification. Bone marrow aspirate smear shows extensive involvement by lymphoma with a predominance of cells having prolymphocytic features (c 1000x magnification). Hematoxylin and eosin-stained slides of bone marrow core biopsy show extensive marrow involvement by lymphoma ((d) 200x and (e) 500x magnification) with lymphoma cells being diffusely positive for cyclin D1 protein (f) 500x magnification.

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