Blastoid Variant Mantle Cell Lymphoma Expressing Aberrant CD3 and CD10 with Concurrent Small Lymphocytic Lymphoma: Establishment of a Clonal Relationship by B- and T-Cell Receptor Gene Rearrangements

Figure 1

Flow cytometry on the lymph node. (a) The majority of cells express bright CD45 with low side angle light scatter characteristic of lymphoid cells. (b) B-cells expressing CD20 range from small to large in size by forward light scatter. (c, d) The large neoplastic cells are positive for CD5 and negative for surface CD3. Cells showing the brightest intensity for CD3 and CD5 are background T-cells. (e, f) Large cells gated in red express dim CD5, but not surface CD3. The cells staining brightest for CD5 and gated in green are background T-cells and also stain for CD3. (g, h) Most CD20 moderate B-cells coexpressing CD5 gated in red also express CD10, while most CD20 dim B-cells gated in green do not express CD10. (i) Most of the CD20 moderate B-cells in red show moderate surface expression of kappa light chain. (j) Most of the CD20 dim B-cells in green do not show any surface light chain expression.

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