Bone Marrow Aspirate Clot: A Useful Technique in Diagnosis and Follow-Up of Hematological Disorders

Figure 2

Case 1. The patient with final diagnosis of megaloblastic hemopathy (pernicious anemia). (a) Microscopic panoramic image to show the general aspect of BMC (H&E, 10x). Each grouping of hematopoietic material is called spicule. Five spicules are necessary to consider a material representative for analysis. The utilized criteria to render MC are the same used to interpret BMB. (b) Erythroid hyperplasia with numerous megaloblasts (H&E, 400x). (c) Demonstration of histochemical staining application on MC (Perls, 400x). In the image, hemosiderin deposits are stained blue. (d) Application of reticulin histochemical staining on MC (reticulin, 400x). Reticulin fibers are stained black. It is possible to evaluate this network with this technique similarly to BMB.

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