Sézary Syndrome with CD4/CD8 Double-Negative Neoplastic T Cells in Peripheral Blood

Figure 4

Results of multicolor flow cytometry of PB cells. After discriminating for dead cells and CD45^– cells, the remaining cells were gated on CD3⁺, and then three T-lymphocyte fractions, CD4⁺CD8^–, CD4^–CD8^–, and CD4^–CD8⁺, were analyzed for CD7 and CD26 expression. Percentages of each cell fraction are indicated in the plot areas. Abbreviations: FSC-A, forward scatter area; SSC-A, side scatter area; 7-AAD, 7-aminoactinomycin D.