Sézary Syndrome with CD4/CD8 Double-Negative Neoplastic T Cells in Peripheral Blood

Figure 5

Results of TCR β-chain gene clonality assays. PCR products were produced and analyzed in accordance with the BIOMED-2 guidelines. The results of capillary electrophoresis of PCR products derived from PB (left) and skin (right) samples are shown. (a), (b), and (c) indicate the data of Vβ + Jβ1/2, Vβ + Jβ2, and Dβ + Jβ1/2, respectively. In each figure, an upper figure shows data from negative control and a lower shows data from sample. The vertical line shows fluorescence intensity, the horizontal line shows fragment size, red arrows show clonal peak, and blue arrows show non-specific peak.