Histology and electron microscopy. (a) Normal liver lobule with pale hepatocytes and ceroid pigments (CP) at their apical pole (H&E staining, original magnification ×400). (b) Diffuse clarification of hepatocytes in relation with marked glycogen reduction (PAS staining). (c) Electron microscopic appearance of a typical hepatocyte showing glycogen deficit (white areas). (d) Overview of the cytoplasm of a typical hepatocyte at electron microscopy. Low density of rough endoplasmic reticulum, mitochondria, and primary lysosomes. Increased number and hyperplasia of smooth endoplasmic reticulum (SER) and Golgi (G) apparatus (white box). Secondary lysosomes (SL) considered in that case as autophagosomes.