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Case Reports in Medicine
Volume 2010, Article ID 541061, 4 pages
http://dx.doi.org/10.1155/2010/541061
Case Report

Screening for Y Chromosome Microdeletion in a Nonobstructive Azoospermic Male Patient with Allogeneic Bone Marrow Transplantation from His Sister

1Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, 34390 Istanbul, Turkey
2Section of Andrology, Department of Urology, Istanbul Faculty of Medicine, Istanbul University, 34390 Istanbul, Turkey

Received 23 September 2010; Revised 22 October 2010; Accepted 25 November 2010

Academic Editor: Mamede de Carvalho

Copyright © 2010 Hakan Gurkan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Genomic DNA of a patient diagnosed with nonobstructive azoospermia and with the history of allogenic bone marrow transplantation from his sister due to chronic myeloid leukemia was isolated from peripheral blood in order to screen Y chromosome microdeletions. 13 short tagged sites belonging to AZF a, b, and c loci were detected with multiplex polymerase chain reaction technique. Bands were determined in ZFX/ZFY wells, whereas no bands were determined in wells of other STS regions. DNA isolation was done from buccal mucosa smear to obtain genomic DNA from patient's own cells and multiplex polymerase chain reaction technique was performed again. Bands were seen in all wells of 13 STS regions. Y chromosome microdeletion was not detected in the patient. In conclusion, genomic DNA isolation in patients undergoing BMT should be done from patients' own cells.