Serological Findings in a Child with Paroxysmal Cold Haemoglobinuria

Figure 3

Indirect Donath-Landsteiner test. Tube 1: OP + red cells suspension + patient’s serum. Double incubation at 0°C and 37°C. Tube 2: duplicate of tube 1 but conserved strictly at 37°C. Tube 3: duplicate of tube 1 + compatible fresh normal EDTA-plasma and double incubation. Tube 4: duplicate of tube 1 + compatible fresh normal serum and double incubation. Tube 5: OP + red cells suspension + ABO compatible fresh serum and double incubation.