Figure 4: Analysis of human goosecoid (GSC) gene mutation in the 3 GSC exons. All 3 exons of the GSC gene were screened in this patient by sequence analysis using the fluorescent dideoxy terminator method and analyzed on an ABI 377 sequencer (Applied Biosystems). Intronic primers flanking each entire exon were designed from human genomic sequence (accession number AL121612) as described previously [9].