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Case Reports in Oncological Medicine
Volume 2013 (2013), Article ID 903094, 3 pages
http://dx.doi.org/10.1155/2013/903094
Case Report

Cell Based Autologous Immune Enhancement Therapy (AIET) after Radiotherapy in a Locally Advanced Carcinoma of the Cervix

1Chennai Meenakshi Multispeciality Hospital Limited, New No. 72, Old No. 148, Luz Church Road, Mylapore, Chennai 600 004, India
2Dr. Kamakshi Memorial Hospital, 1 Radial Road, Pallikaranai, Chennai 600100, India
3Nichi-In Centre for Regenerative Medicine (NCRM), PB 1262, Nungambakkam, Chennai 600034, India
4Biotherapy Institute of Japan, No. 8, No. 4 Edagawa 2-chome, Koto-ku, Tokyo 135-0051, Japan
5Hope Foundation (Trust), B6, 13, Zakariah Colony III Street, Choolaimedu, Chennai 600094, India
6School of Medicine, Yamanashi University, 1110 Shimokato, Chuo, Yamanashi 409-3898, Japan
7The Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM), PB 1262, Nungambakkam, Chennai, Tamil Nadu 600034, India

Received 16 February 2013; Accepted 19 March 2013

Academic Editors: K. Aogi, J. M. Buchanich, S. B. Chichareon, C. Gennatas, and F. A. Mauri

Copyright © 2013 Sumana Premkumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Radiotherapy is the primary form of treatment in patients with locally advanced cervical carcinoma. However for residual disease in the form of the persistent lymph nodes, surgery or chemotherapy is recommended. As surgery is not acceptable by every patient and chemotherapy has associated side effects, we hereby report the positive outcome of in vitro expanded natural killer cell and activated T lymphocyte based autologous immune enhancement therapy (AIET) for the residual lymphadenopathy in a patient with locally advanced cervical cancer after radiation. After six transfusions of AIET, there was complete resolution of residual lymph nodes and there was no evidence of local lesion. The patient also reported improvement in quality of life. As AIET has been reported as the least toxic among the available therapies for cancer, combining AIET with conventional forms of therapy in similar patients might not only improve the outcome but may also help the patients achieve a good quality of life.

1. Introduction

Cancer Statistics in 2008 place cervical cancer as the third most common cancer in women with more than 85% of the global burden occurring in developing countries [1]. There are 529,512 new cases of cervical cancer diagnosed per year globally and 86% of all deaths caused by cervical cancer occur in developing countries [2]. Currently radiotherapy remains the primary treatment of choice for advanced cervical carcinoma patients [2]. Autologous cell based immunotherapy represents one of the potential treatment options for several types of cancers, with randomized clinical trials published on its safety and efficacy [35]. We have earlier reported the positive outcome following autologous immune enhancement therapy (AIET) in pancreatic cancer [6] and ovarian cancer [7] patients. Herein we report the outcome of AIET following radiotherapy in a Stage IV-A cervical cancer patient of Indian origin.

2. Case Presentation

A 58-year-old female patient presented with pain in abdomen and urinary incontinence with hematuria in April 2012 for which she was evaluated. Past medical history revealed that she had bilateral hydronephrosis for which she had undergone stenting. She also had Parkinson’s disease and rheumatoid arthritis for which she was under regular medical management. A CT scan was taken which revealed a large ill-defined irregular mass in the uterine cervix with bilateral parametrial infiltration. The mass measured 5 × 4 cm at its widest point, infiltrating the lower uterine myometrium, extending anteriorly infiltrating the posterior wall and trigone of the bladder involving both vesicoureteric junctions. Posteriorly, the mass had infiltrated the anterior wall of rectum. Few enlarged internal ileac nodes were present bilaterally. Biopsy revealed the mass to be squamous cell carcinoma. She also had bilateral L5 spondylosis with grade II anterolisthesis of L5 over S1. She was treated with external radiation to the pelvis with a dose of 6000 cGy in May 2012. After radiotherapy, the PET-CT in June 2012 (Figure 1(a)) showed residual cervical thickening and residual retroperitoneal lymphadenopathy with increased F18-FDG uptake in the aortocaval and retrocaval lymphnodes with the largest measuring 1.5 × 1.4 cm. Degenerative changes were also noted in the spine. For the residual lymphadenopathy, considering the factors like invasive nature of the surgery and the comorbid conditions of the patient along with fragile nature of her physique making chemotherapy, a high-risk one, the treating physicians recommended autologous natural killer (NK) cell and activated T lymphocyte based immunotherapy termed as autologous immune enhancement therapy (AIET). The first AIET cell transfusion was done in July 2012 and the second and third cell transfusions in August 2012. For each transfusion, approximately 70 mL of peripheral blood was withdrawn and the isolation, in vitro expansion cum activation of the NK cells and T lymphocytes were done as reported earlier [8]. After the three transfusions, an MRI was taken in September 2012 which showed regression of the aortocaval node to 1.4 × 1 cm (earlier 1.5 × 1.4 cm) and the retrocaval node measured 0.9 × 0.5 cm. The fourth and fifth AIET transfusions were done in October 2012 and the sixth transfusions in November 2012. An average of 282.5 million NK cells and 478.5 million activated T lymphocytes were transfused in these six AIET cycles. A PET-CT scan in November 2012 after the completion of all the six cycles of AIET showed complete resolution of the retroperitoneal lymphnodes with no evidence of local lesion in the cervix (Figure 1(b)). The PET-CT also showed sclerotic lesion in the C7 vertebral body. However biopsy of the lesion was not done and the patient is still under followup. In addition to the above changes, the patient also reported improvement in quality of life after the AIET. There were no adverse reactions reported after AIET.

fig1
Figure 1: PET-CT images (a) before and (b) after immunotherapy showing the resolution of the lymph node lesions after immunotherapy.

3. Discussion

Conventional therapies for cervical cancer include radical surgery, radiotherapy, and chemotherapy. Radiotherapy continues to be the preferred form of therapy for advanced cervical cancer [2]. Earlier studies report a median progression free survival of nearly 10 months and overall survival of 21 months in Stage IV-A cervical cancer patients with radiotherapy alone [9]. It has been reported that pelvic lymph node involvement persists in about 16% of the cervical cancer patients after chemoradiation [10]. As lymph node involvement is an important prognostic feature in cervical cancer [11], treatment aimed at removing the lymph nodes involved or controlling the lymph nodal metastasis becomes essential. Usually surgery or chemotherapy is the treatment of choice in such cases. These therapies are associated with potential toxic/side effects. As the patient also had comorbid conditions apart from the above issues of concern, AIET was recommended. After six cycles of AIET, there is a complete resolution of the disease in the lymph nodes involved. The residual thickening observed immediately after the radiation also resolved and there were no adverse effects during the transfusions. As this cell based AIET has been safe and the least toxic of cancer therapies similar to what is reported earlier [12], combining immunotherapy with conventional therapies is suggested for similar cases of locally advanced cervical cancer for early achievement of positive treatment outcome. Further followup of the patient will reveal the significance of immunotherapy in terms of disease free interval and long-term survival benefits.

4. Conclusion

NK cell and activated T lymphocytes based AIET following radiotherapy in our experience with this case did not have any adverse reaction and has resulted in resolution of the residual metastatic lymphadenopathy in a locally advanced cervical carcinoma, and the patient’s general condition is continuing to be optimal throughout. Hence combining AIET with radiotherapy or other conventional therapies could be worth considering for similar patients in the future.

Conflict of Interests

The authors declare that they have no conflict of interests.

Acknowledgments

The authors thank Mr. Ramalingam Karthick and Mr. Mathaiyan Rajmohan for their technical assistance, M/S Hope Foundation and M/S Chennai Cell Cluster for technical advice, Loyola ICAM College of Engineering Technology (LICET) and Loyola Institute of Frontier Energy (LIFE) for their support to their research work, Professor Masaru Iwasaki, Department of Clinical Research, Yamanashi University, Japan, for his advice, Ms. Eiko Amemiya, II Department of Surgery, Yamanashi University School of Medicine, Japan, for her secretarial assistance with the publication of the paper, and Mr. P. Ruban Rex, Centre for Biotechnology, Anna University, for his critical review of the paper.

References

  1. “Globocan 2008 cancer fact sheet,” 2013, http://globocan.iarc.fr/factsheets/cancers/cervix.asp.
  2. L. Denny, “Cervical cancer: prevention and treatment,” Discovery Medicine, vol. 14, no. 75, pp. 125–131, 2012. View at Google Scholar
  3. T. Takayama, T. Sekine, M. Makuuchi et al., “Adoptive immunotherapy to lower postsurgical recurrence rates of hepatocellular carcinoma: a randomised trial,” Lancet, vol. 356, no. 9232, pp. 802–807, 2000. View at Publisher · View at Google Scholar · View at Scopus
  4. H. Kimura and Y. Yamaguchi, “A phase III randomized study of interleukin-2 lymphokine-activated killer cell immunotherapy combined with chemotherapy or radiotherapy after curative or noncurative resection of primary lung carcinoma,” Cancer, vol. 80, no. 1, pp. 42–49, 1997. View at Google Scholar
  5. K. Kono, A. Takahashi, F. Ichihara et al., “Prognostic significance of adoptive immunotherapy with tumor-associated lymphocytes in patients with advanced gastric cancer: a randomized trial,” Clinical Cancer Research, vol. 8, no. 6, pp. 1767–1771, 2002. View at Google Scholar · View at Scopus
  6. S. Baskar, V. Dedeepiya, H. Terunuma et al., “Prolonged survival of a patient with inoperable, locally advanced adenocarcinoma of pancreas after autologous immune enhancement therapy (AIET) with chemotherapy,” Indian Journal of Cancer. In press.
  7. S. R. Manjunath, G. Ramanan, V. D. Dedeepiya et al., “Autologous immune enhancement therapy in recurrent ovarian cancer with metastases: a case report,” Case Reports in Oncology, vol. 5, no. 1, pp. 114–118, 2012. View at Google Scholar
  8. M. Takada, H. Terunuma, X. Deng et al., “Refractory lung metastasis from breast cancer treated with multidisciplinary therapy including an immunological approach,” Breast Cancer, vol. 18, no. 1, pp. 64–67, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. P. G. Rose, S. Ali, C. W. Whitney, R. Lanciano, and F. B. Stehman, “Outcome of stage IVA cervical cancer patients with disease limited to the pelvis in the era of chemoradiation: a Gynecologic Oncology Group study,” Gynecologic Oncology, vol. 121, no. 3, pp. 542–545, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. G. Houvenaeghel, L. Lelievre, A. L. Rigouard et al., “Residual pelvic lymph node involvement after concomitant chemoradiation for locally advanced cervical cancer,” Gynecologic Oncology, vol. 102, no. 1, pp. 74–79, 2006. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Polterauer, L. Hefler, V. Seebacher et al., “The impact of lymph node density on survival of cervical cancer patients,” British Journal of Cancer, vol. 103, no. 5, pp. 613–616, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Goto, N. Shirotani, M. Hatakeyama et al., “Clinical benefit of non-toxic therapy in patients with advanced cancer (opinion),” Anticancer Research, vol. 22, no. 4, pp. 2461–2464, 2002. View at Google Scholar · View at Scopus