Clinical Application of Liquid Biopsy in Targeted Therapy of Metastatic Colorectal Cancer

Figure 2

Dynamics of a KRAS mutant clone in plasma samples of the patient in case 2. The red line indicates the frequency of KRAS exon 3 mutation (percentage of alleles) detected in circulating DNA at the indicated time points. Progressive disease occurred after the long-term treatment with anti-EGFR antibodies. After interruption of anti-EGFR therapy; the KRAS mutation load distinctly declined and remained below the limit of detection across subsequent lines of treatment. “E” represents therapy with anti-EGFR antibodies and chemotherapy and “V” therapy with anti-VEGF antibodies and chemotherapy; “PHE” acts for partial hepatectomy and “m” for month. Results of follow-up are indicated below the graphs: green box, partial remission; yellow box, stable disease; and red box, progressive disease.