Residual NADPH Oxidase Activity and Isolated Lung Involvement in X-Linked Chronic Granulomatous Disease
Figure 4
Dihydrorhodamine-123 (DHR) fluorescence assay in peripheral-blood neutrophils from the patient and an unrelated healthy control are displayed. In this assay, neutrophils are stimulated with phorbol myristate acetate (PMA) in the presence of DHR, and flow cytometry is used to measure the oxidative burst mediated by NADPH oxidase by quantifying the fluorescent product. After PMA stimulation, the histogram from the control (a) shows a unimodal shift of fluorescence far to the right, whereas the patient has a broad-based shift in fluorescence that is only partially shifted to the right compared with the healthy control (b).
