Figure 1: Supernatant cytotoxicity of Clostridium sordellii isolate. Culture supernatant (48 h) C. sordellii was incubated with an ~50% confluent Vero cell monolayer in a 96-well plate in a total of 100 μL per well and observed for 2 hr for cytopathic effects (CPE). Pictures shown are representative of a routine cell treatment with the indicated supernatant dilution, and cells were observed at a magnification of ×40. As a negative control, culture supernatant was heated to 90°C for 10 min and cooled prior to addition to cells. The degree of cytopathic effect was plotted against the relative dilution of the culture supernatant and is representative of eight independent trials.