Figure 2: Analysis of sordellilysin in Clostridium sordellii isolates. Primers designed to amplify cholesterol dependent cytolysins (cdc) based on the reported sequence of perfringolysin O (pfo) were included in PCR reactions for each C. sordellii isolate. Amplification products were separated by 0.8% agarose gel electrophoresis and visualized via ethidium bromide staining. Lanes are designated by the strain names of each isolate in the figure. C. sordellii ATCC9714 was used as a positive control from sordellilysin expression.