ELISA-Based Crossmatching Allowing the Detection of Emerging Donor-Specific Anti-HLA Antibodies through the Use of Stored Donors’ Cell Lysates

Table 3

(a) HLA typing results of the patient, his donor from 1998, and a refused kidney offer from 2009. Identifiable HLA-specific antibodies and crossmatch-ELISA results of the patient are presented. (b) AbCross-crossmatch-ELISA results using three sera of the patient (taken in 06/2009, 07/2011, and 12/2012) which were tested for DSA against three selected stored leukocyte pellet lysates of virtual donors bearing the allele HLA-A25:01.

(a)

Patient’s typing	Donor’s typing (1998)	Typing of the refused kidney offer (2009)
A3,25 (10)	A3,25 (10)	A3,25 (10)
	(high res. not done)
B8,18 (Bw6)	B8,18 (Bw6)	B18,49 (Bw4,6)
Cw7,12	Cw7,12	Cw7,12
DR15,17	DR15,15	DR4,14
DR51,52	DR51,51	DR52,53
DQ2,6	DQ6,6	DQ3 (8),5
Patient’s identified anti-HLA antibodies: anti-A25 (10); other HLA-A10 specifications: anti-A26 (10), anti-A34 (10), and anti-A66 (10).

Crossmatch results using the patient’s pretransplant serum from 1998: negative CDC-based pre-TX crossmatch in 1998 with PBL, T-cell, and B-cell.
Crossmatch results using the patient’s pretransplant serum from 06/2009: positive CDC-based pre-TX crossmatch in 2009 with PBL, T-cell, and B-cell.

(b)

Patient’s serum sample	AbCross-ELISA-CM results against; selected virtual donors
	B.H.	K.P.	T.H.
	class I/II	class I/II	class I/II
06/2009	pos./neg.	pos./neg.	pos./neg.
07/2011	pos./neg.	pos./neg.	pos./neg.
12/2012	pos./neg.	pos./neg.	pos./neg.
Virtual donors’ HLA-A high res. typing results: B.H.: HLA-A24:02, 25:01, K.P.: HLA-A25:01, 26:01, T.H.: HLA-A02:01, 25:01.