Mesothelioma in Two Nondomestic Felids: North American Cougar (Felis concolor) and Cheetah (Acinonyx jubatus)
Figure 3
Photomicrographs of a mass overlying the epicardium and main pulmonary artery from a 15-year-old cougar. (a) Multiple papillary structures of fibrovascular stroma and nests of anisocytotic pleomorphic polygonal cells are covered by pleomorphic anisocytotic cuboidal to columnar cells (hematoxylin and eosin stain). (b) Clustered and individual cells strongly stain brown for the presence of cytokeratin intermediate filaments (immunohistochemistry, cytokeratin [AE1/AE3] antibody, DAB chromagen, hematoxylin counterstain). (c) The same clustered and individual cells stain brown for the presence of vimentin intermediate filaments (immunohistochemistry, vimentin [V9] antibody, DAB chromagen, hematoxylin counterstain). All figures are at 40x objective. Bar = 50 μM.
