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Case Reports in Veterinary Medicine
Volume 2013 (2013), Article ID 318548, 9 pages
http://dx.doi.org/10.1155/2013/318548
Case Report

Two Cases of Lacaziosis in Bottlenose Dolphins (Tursiops truncatus) in Japan

1General Research Center, Okinawa Churaumi Aquarium, Aza Ishikawa 888, Motobu-Cho, Kunigami-Gun, Okinawa 905-0206, Japan
2Department of Animal Sciences, Faculty of Agriculture, University of the Ryukyus, Sembaru 1, Nishihara-Cho, Nakagusuku-Gun, Okinawa 903-0213, Japan
3Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Rinku-Campus, Osaka Prefecture University, Rinku Ohrai Kita 1-58, Izumisano, Osaka 598-8531, Japan
4Department of Pathological Science, CCB, State University of Londrina, P.O. Box 6001, 86051-970 Londrina, PR, Brazil
5Medical Mycology Research Center, Chiba University, Inohana 1-8-1, Chiba 260-8673, Japan
6Department of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai Midorimachi 582, Ebetu, Hokkaido 069-0836, Japan

Received 21 June 2013; Accepted 4 August 2013

Academic Editors: J. Lakritz, F. Mutinelli, R. L. Santos, and S. Stuen

Copyright © 2013 Keiichi Ueda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lacaziosis, formerly called lobomycosis, caused by Lacazia loboi, is a zoonotic mycosis found in humans and dolphins and is endemic in the countries on the Atlantic Ocean. Although the Japanese coast is not considered an endemic area, photographic records of lacaziosis-like skin lesions were found in bottlenose dolphins (Tursiops truncatus) that were migrating in the Goto Islands (Nagasaki Prefecture, Japan). We diagnosed 2 cases of lacaziosis in bottlenose dolphins captured simultaneously at the same coast within Japanese territory on the basis of clinical characteristics, cytology, histopathology, immunological tests, and detection of partial sequences of a 43 kDa glycoprotein coding gene (gp43) with a nested-PCR system. The granulomatous skin lesions from the present cases were similar to those found in animals from endemic areas, containing multiple budding and chains of round yeast cells and positive in the immune-staining with anti-Paracoccidioides brasiliensis serum which is a fungal species related to L. loboi; however, the gp43 gene sequences derived from the present cases showed 94.1% homology to P. brasiliensis and 84.1% to L. loboi. We confirmed that the causative agent at the present cases was different genotype of L. loboi from Amazon area.