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Canadian Respiratory Journal
Volume 2018, Article ID 5259240, 8 pages
https://doi.org/10.1155/2018/5259240
Research Article

Budesonide and Calcitriol Synergistically Inhibit Airway Remodeling in Asthmatic Mice

Department of Pediatrics, Wuxi Children’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu, China

Correspondence should be addressed to Zhiwei Yu; moc.nuyila@40289uy

Received 15 January 2018; Revised 25 March 2018; Accepted 4 April 2018; Published 2 May 2018

Academic Editor: Jörg D. Leuppi

Copyright © 2018 Jun Qian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background and Objective. While calcitriol can inhibit airway remodeling in asthmatic mice, the mechanism remains unclear. The purpose of this study was to explore the mechanism of action of calcitriol on airway remodeling in asthma and its interaction with budesonide. Methods. A mouse model of asthma was established by allergic sensitization and challenge with ovalbumin. The mice were treated with budesonide, calcitriol, or budesonide plus calcitriol. The expression of airway remodeling-related proteins, transforming growth factor β (TGFβ) signaling pathway-related proteins, the glucocorticoid receptor, and vitamin D receptor (VDR) was determined by immunohistochemical staining and Western blot analysis. Quantitative real-time PCR was used to determine the expression of microRNA-21 (miR-21) in the lung tissue of mice. Results. Monotherapy with budesonide or calcitriol inhibited the high expression of collagen type I protein and upregulated the low expression of Smad7 in asthmatic mice. There was a synergistic interaction between budesonide and calcitriol in combined treatment. The expression of miR-21 in the combined treatment group was significantly lower than that in the calcitriol treatment group. VDR expression in the combined treatment group was significantly higher than that of the calcitriol treatment group. Conclusion. Budesonide and calcitriol have a synergistic effect on airway remodeling in asthmatic mice.