Figure 2: Effect of budesonide (Bud) and calcitriol (Cal) on the expression of airway remodeling-related proteins in ovalbumin- (Ova-) treated mice. (a) The lung tissue of the mice was fixed, embedded, and sectioned for immunohistochemical staining (DAB stain, original magnification ×400). (b) The total protein was extracted from the lung tissue of mice for Western blot analysis. (c, d, e) The expression of COL I, COL III, and α-SMA in the lung tissue of mice was determined by Western blot analysis, with GAPDH as an internal reference ( versus control group, versus Ova-treated group).