Overexpression of MALAT1 Relates to Lung Injury through Sponging miR-425 and Promoting Cell Apoptosis during ARDS

Figure 2

MALAT1 directly interacting with miR-425 and up-regulated PTEN expression through sponging miR-425. (a) Schematic diagram of predicted interaction between MALAT1 and miR-425. (b) Dual luciferase assay. A549 and HFL-1 cells were transfected with MALAT1 reporter vector and miR-425 mimic or control oligo for 48 hours. Cells were lysed and subjected to luciferase assay. Results were analyzed by Student’s t-test, and was considered significant. (c) A549 and HFL-1 cells were transfected with wildtype or mutant MALAT1 expression vector, with empty vector as control. The MALAT1 level was detected 48 hours after transfection. MiR-425 level in the cell culture medium was detected by qRT-PCR at the same time (d). (e) A549 and HFL-1 cells were transfected with MALAT1 specific siRNAs for 48 hours. The MALAT1 level in A549 and HFL-1 was detected by qRT-PCR. (f) The miR-425 level in the medium was quantified by qRT-PCR. Results were analyzed by t-test, and was considered significant. , .

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