Overexpression of MALAT1 Relates to Lung Injury through Sponging miR-425 and Promoting Cell Apoptosis during ARDS

Figure 3

Exosome from ARDS patients’ plasma delivered less miR-425 into target cells and did not repress PTEN expression. (a) A549 and HFL-1 cells were cocultured with exosomes from ARDS patients or healthy controls. The miR-425 level was detected by qRT-PCR 48 hours after coculture. (b) A549 and HFL-1 cells were transfected with PTEN reporter and then cocultured with exosomes from ARDS patients or healthy controls. The luciferase activity was detected 48 hours after coculture. Results were analyzed by t-test, and was considered significant. , . (c) A549 and HFL-1 cells were cocultured with exosomes from ARDS patients or healthy controls. The PTEN protein level was determined by immunoblotting.

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