LASP1 Induces Epithelial-Mesenchymal Transition in Lung Cancer through the TGF-β1/Smad/Snail Pathway

Figure 6

(a–c) LASP1 effect on nuclear levels of key molecules in the TGF-β1/Smad/Snail signaling pathway in A549 and SK-MES-1 cell lines under TGF-β1 treatment. After 48 h coinfection with TGF-β1 accompanying LASP1 knockdown or overexpression, the nuclear fractions were detected for protein levels of pSmad2, pSmad3, and Snail1 by western blot. (a) α-Tubulin (cytoplasmic marker) and Lamin B (nuclear marker) demonstrated the purified nuclear extracts. The representative images of western blot and the quantification normalized with (b) Lamin B in A549 and (c) SK-MES-1 cells. Data were shown as mean ± SD, n = 3. and vs. the cell alone group without TGF-β1 treatment, and , , and vs. the TGF-β1-treated noninfection group (TGF-β1).

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