Inhibition of MAD2L1 Mediates Pulmonary Fibrosis through Impairment of Mitochondrial Function and Induction of Cell Senescence

Figure 4

MAD2L1 inhibition impaired mitochondrial structure and function. (a) The MMP levels of A549 cells transfected with MAD2L1 siRNA or control siRNA were measured by JC-1 staining. (c) The MMP levels of A549 cells were detected by JC-1 staining following treatment with M2I-1 or DMSO (control). (b, d) Quantitative analysis of the MMP percentage. The MMP percentage was calculated as the ratio of JC-1 aggregates to monomers. (e) The expression of MFN1, MFN2, OPA1, and PINK1 was determined by western blot analysis. β-actin was used as the loading control. (f) Western blot analysis was performed to confirm the alterations in MFN1, MFN2, OPA1, and PINK1 in total protein extracts after treatment with M2I-1 or DMSO (control).

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