Inhibition of MAD2L1 Mediates Pulmonary Fibrosis through Impairment of Mitochondrial Function and Induction of Cell Senescence

Figure 6

Inhibition of MAD2L1 augmented the senescence of A549 cell. (a) Cell senescence was detected by SA-β-Gal staining following transfection with MAD2L1 siRNA or control siRNA. (b) The data are presented as the % of β-gal-positive cells/total cells. (c) SA-β-Gal staining was applied to detect cellular senescence following treatment with the MAD2L1 inhibitor M2I-1 or control. (d) The data are presented as the % of β-gal-positive cells/total cells. (e) The expression of MAD2L1 and aging-related marker proteins (serine-20-phosphorylated p53, p53, threonine-145-phosphorylated p21, and p21) were determined by western blot analysis. β-actin was used as the control. (f) Western blot analysis was performed to confirm the alterations in serine-20-phosphorylated p53, p53, threonine-145-phosphorylated p21, and p21. β-actin was used as the control. ; .

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