Inhibition of MAD2L1 Mediates Pulmonary Fibrosis through Impairment of Mitochondrial Function and Induction of Cell Senescence

Figure 7

MAD2L1 inhibition increased the synthesis of extracellular matrix proteins. (a) The expression of E-cadherin and the ECM-related proteins Collagen1, N-cadherin, α-SMA, and Vimentin was detected by western blot analysis in A549 cells transfected with MAD2L1 siRNA or control siRNA. (b) The expression of E-cadherin and the ECM-related genes Collagen1, N-cadherin, α-SMA, and Vimentin were determined by qRT-PCR in A549 cells transfected with MAD2L1 siRNA or control siRNA. (c) qRT-PCR analysis of Collagen1, E-cadherin, N-cadherin, α-SMA, and Vimentin mRNA expression in A549 cells treated with M2I-1 or DMSO (control). (d) Western blot analysis was performed to confirm the alterations in Collagen1, E-cadherin, N-cadherin, α-SMA, and Vimentin in total protein extracts from cells treated with M2I-1 or DMSO (control).

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