Locally Different Endothelial Nitric Oxide Synthase Protein Levels in Ascending Aortic Aneurysms of Bicuspid and Tricuspid Aortic Valve
Table 2
Protein levels of 35 apoptosis-related proteins after treatment of VSMCs with 1 mM L-NAME.
Protein
Control average
1 mM L-NAME average
Change
P
Bad
50.59 ± 8.08
54.73 ± 9.86
4.15
0.637
Bax
47.65 ± 14.26
50.98 ± 13.43
3.32
0.805
Bcl-2
31.37 ± 7.47
34.32 ± 5.04
2.95
0.633
Bcl-x
17.62 ± 4.92
19.02 ±6.20
1.40
0.780
Pro-Caspase 3
107.21 ± 20.89
109.96 ± 19.89
2.75
0.889
Cleaved Caspase 3
40.89 ± 12.79
43.35 ± 6.22
2.46
0.799
Catalase
93.40 ± 24.75
97.04 ± 25.26
3.63
0.880
clAP-1
39.80 ± 20.28
45.09 ± 20.87
5.28
0.792
clAP-2
16.72 ± 4.71
18.86 ± 5.27
2.15
0.630
Claspin
40.49 ± 6.13
43.37 ± 6.39
2.89
0.618
Clusterin
22.70 ± 6.30
27.93 ± 7.19
5.23
0.437
Cytochrome c
68.46 ± 8.61
76.39 ± 8.48
7.93
0.336
TRAIL R1/DR4
40.74 ± 6.48
46.36 ± 3.04
5.62
0.241
TRAIL R2/DR5
66.06 ± 16.01
77.34 ± 6.60
11.28
0.363
FADD
64.20 ± 13.56
71.59 ± 4.95
7.39
0.467
Fas/TNFSF6
66.16 ± 23.27
68.26 ± 13.45
2.10
0.904
HIF-1a
29.40 ± 16.62
41.97 ± 15.61
12.57
0.436
HO-1/HMOX1/HSP32
27.06 ± 14.68
35.64 ± 12.58
8.58
0.526
HO-2/HMOX2
38.85 ± 14.23
49.90 ± 18.08
11.04
0.497
HSP27
33.21 ± 7.16
38.64 ± 9.88
5.43
0.528
HSP60
65.84 ± 11.85
70.83 ± 7.05
4.99
0.545
HSP70
66.75 ± 13.76
64.40 ± 5.59
−2.35
0.801
HTRA2/Omi
63.09 ± 3.83
72.57 ± 4.18
9.48
0.038*
Livin
14.08 ± 2.52
18.83 ± 5.12
4.75
0.223
PON2
28.81 ± 5.92
31.30 ± 5.53
2.49
0.651
p21/CIP1/CDNK1A
27.93 ± 9.01
38.74 ± 9.16
10.81
0.259
p27/Kip1
16.75 ± 6.04
21.34 ± 2.89
4.59
0.345
Phospho-p53 (S15)
82.25 ± 19.34
88.18 ± 11.03
5.93
0.696
Phospho-p53 (S46)
64.83 ± 18.94
71.42 ± 20.27
6.59
0.729
Phospho-p53 (S392)
64.02 ± 28.92
75.33 ± 22.81
11.31
0.659
Phospho-Rad17 (S635)
11.71 ± 2.21
18.72 ± 5.65
7.01
0.129
SMAC/Diablo
56.47 ± 11.12
59.84 ± 9.98
3.36
0.742
Survivin
57.52 ± 23.63
61.96 ± 19.33
4.44
0.831
TNF RI/TNFRSF1A
18.18 ± 4.27
22.05 ± 1.64
3.87
0.210
XIAP
49.96 ± 5.39
56.28 ± 3.45
6.32
0.149
All values are in percent of the calibrator (positive control). The change is given in percent points of the calibrator. The data were acquired in three independent experiments. *: P < 0.05.
