Locally Different Endothelial Nitric Oxide Synthase Protein Levels in Ascending Aortic Aneurysms of Bicuspid and Tricuspid Aortic Valve
Table 3
Protein levels of 35 apoptosis-related proteins after treatment of HAECss with 1 mM L-NAME.
Protein
Control average
1 mM L-NAME average
Change
P
Bad
62.83 ± 4.88
66.76 ± 12.67
3.92
0.661
Bax
52.36 ± 16.36
54.62 ± 12.14
2.26
0.878
Bcl-2
35.77 ± 6.52
40.28 ± 4.95
4.51
0.373
Bcl-x
20.18 ± 5.18
17.03 ± 3.48
−3.16
0.410
Pro-Caspase 3
92.79 ± 10.67
89.73 ± 9.55
−3.06
0.756
Cleaved Caspase 3
29.29 ± 7.84
23.81 ± 3.32
−5.48
0.392
Catalase
58.39 ± 12.35
55.87 ± 6.19
−2.53
0.803
clAP-1
35.59 ± 9.16
27.73 ± 16.97
−7.87
0.560
clAP-2
15.76 ± 3.62
16.86 ± 5.57
1.10
0.813
Claspin
18.69 ± 2.87
18.00 ± 2.51
−0.69
0.745
Clusterin
35.62 ± 11.69
39.72 ± 4.57
4.10
0.660
Cytochrome c
62.37 ± 11.07
84.29 ± 6.92
21.92
0.069
TRAIL R1/DR4
56.01 ± 3.15
57.91 ± 12.80
1.91
0.833
TRAIL R2/DR5
88.48 ± 1.03
91.83 ± 8.18
3.35
0.516
FADD
72.14 ± 8.15
72.21 ± 7.40
0.07
0.989
Fas/TNFSF6
41.58 ± 7.37
34.54 ± 9.89
−7.04
0.303
HIF-1a
28.35 ± 7.46
26.07 ± 6.38
−2.29
0.735
HO-1/HMOX1/HSP32
30.43 ± 6.38
26.90 ± 12.93
−3.53
0.728
HO-2/HMOX2
35.86 ± 1.94
34.72 ± 11.64
−1.15
0.887
HSP27
41.67 ± 9.25
44.93 ± 11.15
3.26
0.699
HSP60
62.68 ± 8.65
70.54 ± 22.29
7.87
0.640
HSP70
65.35 ± 7.42
69.88 ± 17.35
4.53
0.727
HTRA2/Omi
78.98 ± 5.64
84.13 ± 9.00
5.15
0.482
Livin
12.39 ± 2.85
17.58 ± 6.45
5.18
0.315
PON2
41.23 ± 2.47
44.50 ± 12.63
3.27
0.711
p21/CIP1/CDNK1A
30.05 ± 11.24
35.52 ± 9.31
5.47
0.611
p27/Kip1
30.42 ± 3.67
29.70 ± 4.48
−0.73
0.777
Phospho-p53 (S15)
22.92 ± 6.58
25.48 ± 6.03
2.56
0.680
Phospho-p53 (S46)
8.84 ± 4.14
9.08 ± 2.81
0.24
0.948
Phospho-p53 (S392)
4.06 ± 2.69
5.01 ± 6.63
0.95
0.844
Phospho-Rad17 (S635)
12.24 ± 3.83
13.16 ± 9.86
0.92
0.900
SMAC/Diablo
63.31 ± 14.25
63.90 ± 12.04
0.59
0.956
Survivin
23.28 ± 1.48
21.28 ± 3.96
−2.00
0.443
TNF RI/TNFRSF1A
27.19 ± 3.24
25.24 ± 4.74
−1.95
0.599
XIAP
53.37 ± 7.73
51.67 ± 3.93
−1.70
0.769
All values are in percent of the calibrator (positive control). The change is given in percent points of the calibrator. The data were acquired in three independent experiments.
