Nitric Oxide Manipulation: A Therapeutic Target for Peripheral Arterial Disease?

Figure 2

ADMA/DDAH pathway in ischaemic muscle—preliminary data. (a) Western blot showing reduced DDAH2 expression in muscle biopsies from patients with CLI. Lanes 1–4: control, 5–8: CLI muscle. (b) ELISA showing significantly higher ADMA levels in muscle from patients with CLI (𝑃=0.03, Mann Whitney U test). (c) Western blot showing reduced DDAH2 expression in hypoxic myotubes (C: control, H: hypoxia). (d) Conditioned medium from hypoxic C2C12 myotubes contained elevated levels of ADMA compared to medium from myotubes cultured in normoxia. (𝑃<0.05, Student′s t-test). Medium from HMEC-1 cells was used as positive control.

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