Neutralization by Insulin of the Hypertensive Effect of Dermcidin Isoform 2: An Environmentally Induced Diabetogenic and Hypertensive Protein
Figure 6
Effect of insulin on platelet aggregation in platelet rich plasma induced by electrophoretically pure dermcidin. Platelet rich plasma (PRP) was prepared as described in Section 2 and incubated with ≈1.0 nM dermcidin for 90 min at 37°C. In a parallel experiment, PRP was incubated with the same amount of dermcidin in the presence of 100 μunits/mL insulin to determine the effect of the hormone on the dermcidin-induced platelet aggregation. The curves grouped under A represent the aggregation of the PRP incubated with ≈1.0 nM dermcidin in 10 different PRP from 10 different normal volunteers. Curve B shows the inhibition of platelet aggregation incubated with ≈1.0 nM dermcidin and 100 μunits insulin. Curve C represents the platelet aggregation of PRP in control experiment. The figure shown here is a typical representative of at least 10 different experiments using purified dermcidin in PRP from 10 different normal volunteers (; ).
