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Cardiology Research and Practice
Volume 2018, Article ID 5812704, 5 pages
https://doi.org/10.1155/2018/5812704
Research Article

Plasma Chemerin Levels Are Increased in ST Elevation Myocardial Infarction Patients with High Thrombus Burden

1Department of Cardiology, Samsun Training and Research Hospital, University of Health Sciences, Samsun, Turkey
2Department of Microbiology, Gazi State Hospital, Samsun, Turkey
3Department of Cardiology, Ahi Evren Training and Research Hospital, University of Health Sciences, Trabzon, Turkey

Correspondence should be addressed to Uğur Arslan; moc.oohay@5nalsrarugu

Received 19 October 2017; Accepted 6 February 2018; Published 26 March 2018

Academic Editor: Anne Knowlton

Copyright © 2018 Ahmet Hakan Ateş et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To investigate plasma chemerin levels in ST elevation myocardial infarction (STEMI) patients and find out possible relationships between plasma chemerin levels and angiographic characteristics. Patients and Methods. Ninety-seven consecutive patients who presented with STEMI and underwent primary percutaneous coronary intervention (PCI) with coronary stents were enrolled, and 30 age- and sex-matched patients with stable angina pectoris who underwent coronary angiography formed the control group. Angiographic characteristics of the patients including thrombolysis in myocardial infarction (TIMI) thrombus and Gensini scores were noted. Blood samples were taken to detect several biochemical markers including plasma chemerin levels at the admission to hospital. Results. Serum chemerin and C-reactive protein (CRP) levels were significantly increased in patients with STEMI. Among STEMI patients, serum chemerin levels were significantly higher in patients with high thrombus burden (581.5 ± 173.7 versus 451.3 ± 101.2 mg/dL, ). CRP levels and peak creatine kinase-MB (CK-MB) levels were higher, and left ventricular ejection fraction and post-PCI TIMI flow were lower in patients with high thrombus burden. After multivariate analysis, serum chemerin levels were also higher in patients with high thrombus grade (odds ratio: 1.009 (1.005–1.014), ). Besides, serum chemerin levels were also found to be significantly correlated with CRP and peak CK-MB levels. Conclusions. Results from our study have demonstrated for the first time that chemerin levels were higher in STEMI patients with greater thrombus burden and higher level of inflammation.

1. Introduction

Acute ST segment elevation myocardial infarction (STEMI) most commonly occurs when thrombus formation results in complete occlusion of a major epicardial coronary artery. The most serious form of acute coronary syndromes, STEMI is a life-threatening situation, and it must be diagnosed and treated promptly via coronary revascularization, usually by percutaneous coronary intervention or fibrinolytic therapy. Several biomarkers have been studied in STEMI to predict the prognosis of this disease which mainly develops due to an atherosclerotic and thrombotic condition.

Adipose tissue not only serves as a tissue of fat for energy storage, but also behaves like an active endocrine organ secreting cytokines and bioactive adipokines, such as leptin, adiponectin, tumor necrosis factor-alpha (TNF-α), and resistin [13]. Adipokines have several systemic effects on different organs such as liver, brain, vasculature, and endothelium. They have an effect on endothelial cells, arterial smooth muscle cells, and macrophages all of which have different roles in the atherosclerotic process [4].

Chemerin, also known as tazarotene-induced gene 2 protein (TIG2) or retinoic acid receptor responder 2 (RARRES2), is a recently discovered adipocytokine that is released from liver and white fat tissue. It has been shown to regulate adipocyte differentiation, maturation, and metabolism [5, 6], and it modulates the expression of adipocyte genes, such as glucose transporter-4, adiponectin, and leptin. These genes are involved in lipid homeostasis [7, 8]. Several studies have reported that chemerin levels are also associated with inflammation, adipogenesis, components of metabolic syndromes, lipid homeostasis, atherosclerosis, and peripheral arterial stiffness [911]. Circulating chemerin levels are found to be elevated in multiple metabolic and inflammatory diseases such as type 2 diabetes, metabolic syndrome, psoriasis, and cardiovascular diseases [12]. The role of chemerin in pathogenesis and development of cardiovascular disease and atherosclerosis has been investigated in some trials, and a positive correlation between chemerin secretion and coronary atherosclerosis has been shown by Spiroglou et al. [13]. However, negative studies are also present.

Herein, we studied the circulating chemerin levels in patients with STEMI undergoing primary percutaneous coronary intervention (PCI). Our aim was to assess the relationship between chemerin levels and several clinical and angiographic parameters, especially the thrombus burden in patients with STEMI.

2. Methods

2.1. Study Population

We enrolled 97 consecutive patients who presented with STEMI and underwent primary PCI with coronary stents at the Samsun Training and Research Hospital between January 2016 and January 2017. These patients were presented with STEMI within the first 12 hours after the onset of pain and had an ST segment elevation at least 1 mm (2 mm for V1-V3) in two or more contiguous leads in electrocardiogram (ECG) or new onset left bundle-branch block. Patients with cardiogenic shock, admission 12 h after the onset of chest pain, history of renal dysfunction (serum creatinine ≥ 1.5 mg/dL), evidence of malignant disease, or unwillingness to participate were excluded from the study. Control group was constituted from 30 age- and sex-matched patients with stable angina pectoris who underwent coronary angiography. Stable angina pectoris was defined as stable anginal or equivalent symptoms lasting for at least one month.

The characteristics of patients and medical history such as age, sex, history of hypertension, diabetes, hyperlipidemia, and smoking status were recorded. Additionally, serum creatinine, lipid panel, cardiac biomarkers such as creatine kinase-MB (CK-MB), and C-reactive protein (CRP) were measured for all patients during hospital admission. Blood samples for chemerin were taken just before percutaneous coronary intervention at the admission to hospital.

Human Chemerin (CHEMERIN) ELISA kit (Hangzhou Eastbiopharm Co. Hangzhou, China), Synergy 4 Microplate Reader (BioTek, USA), and a Multiwash (TriContinent Scientific, USA) microplate washer were used to measure serum chemerin levels (ng/mL) which were calculated according to a standard curve which was formed using a curvetting equation.

2.2. Clinical and Angiographic Definitions

Hypertension (HTN) was defined as systolic pressure >140 mm Hg and/or a diastolic pressure >90 mm Hg or if the patient was taking antihypertensive medications before the admission to our center. The diagnosis of diabetes mellitus (DM) was defined as a previous history of DM treated with drug therapies or patients with HbA1c concentrations over 6.5% at admission. Hyperlipidemia was defined as total cholesterol level >200 mg/dL or previous history of statin use. Current smokers were defined as those who had smoked for some period during the past year. Left ventricular ejection fraction (LVEF) was measured using the modified Simpson’s method with transthoracic echocardiography, which was performed on the third day of STEMI.

Coronary angioplasty and stent implantation were performed according to standard percutaneous techniques through the femoral artery. Standard selective coronary angiography with at least 4 views of the left coronary system and 2 views of the right coronary artery was performed using the Judgkins technique. All the patients who were admitted with acute STEMI underwent emergent PCI to the culprit vessel with drug-eluting stents.

The Gensini score considering the extent and severity of the lesions at coronary angiography was calculated for each patient [14]. Coronary artery stenosis was measured visually and scored as 1 for 1–25% stenosis, 2 for 26–50% stenosis, 4 for 51–75% stenosis, 8 for 76–90% stenosis, 16 for 91–99% stenosis, and 32 for total occlusion. Then, this score was multiplied by a constant number which was obtained according to the anatomical position of the lesion, and Gensini score was the product of this multiplication.

Angiographic coronary thrombus burden was scored based on 5 grades, and the classification of thrombus burden is demonstrated in Table 1 in detail. We primarily calculated TIMI thrombus grade based on the initial diagnostic angiogram after restoring antegrade flow through guidewire or small balloon dilatation [1517]. We then stratified the final TIMI thrombus grades as low thrombus burden or high thrombus burden, based on scores 1 to 3 or 4 to 5, respectively.

Table 1: The TIMI thrombus burden classificationa.
2.3. Statistical Analysis

The analysis of the results was performed using the Statistical Package for Social Sciences (SPSS, Chicago, Illinois, USA), version 16.0 software for Windows. Data were tested for normal distribution using the Kolmogorov–Smirnov test. The categorical variables were shown as numbers of cases with percentages, and the normally distributed continuous variables were shown as mean ± standard deviation. Median values (minimum-maximum) were used for the continuous variables which were not normally distributed. Student’s t-test was used for the analysis of the continuous variables which were normally distributed, and the χ2 test was used for the categorical variables. A value of 0.05 was considered statistically significant. Pearson and Spearman correlation coefficients were used for correlation analysis where appropriate.

3. Results

The baseline characteristics of the patients are summarized in Table 2. Serum chemerin and CRP levels were significantly increased in patients with STEMI. Besides, Gensini score was higher in STEMI patients among the angiographic parameters.

Table 2: Baseline characteristics of the patients.

In STEMI patients with high thrombus burden, serum chemerin levels were significantly higher than the patients with low thrombus burden (581.5 ± 173.7 versus 451.3 ± 101.2 mg/dL, ). CRP levels and peak CK-MB levels were higher, and LVEF and post-PCI TIMI flow were lower in patients with high thrombus burden (Table 3). Multivariate analysis was performed in these patients, and chemerin levels were still found to be significantly increased in patients with high thrombus grade (OR: 1.009 (1.005–1.014), ).

Table 3: The characteristics of STEMI patients according to their thrombus burden.

When correlation analysis was performed, serum chemerin levels were found to be significantly correlated with CRP levels and peak CK-MB levels . There was no correlation between the serum chemerin levels and Gensini score .

4. Discussion

In this study, the main finding was the significant relationship between circulating chemerin levels and the thrombus burden in patients with STEMI undergoing primary PCI. Besides, increased serum chemerin levels were also associated with increased jeopardized myocardial area which was assessed by peak CK-MB levels and increased level of inflammation determined by CRP levels. To the best of our knowledge, this is the first study in the literature evaluating the relationship between chemerin levels and thrombus burden in STEMI.

Inflammation and lipid hemostasis have been demonstrated to have a pivotal role in the pathogenesis of atherosclerotic coronary artery disease and acute coronary syndromes. The adipokines which are released from the adipose tissue such as chemerin, interleukin 6 (IL-6), leptin, and tumor necrosis factor- (TNF-) α play different roles in the inflammatory process causing development and acceleration of atherosclerosis and contributing to the presence of acute coronary syndromes [1823]. It has been also found that serum chemerin levels are correlated with both arterial stiffness and coronary arterial plaques [11, 13, 24]. Lehrke et al. found a weak relationship about the relation between chemerin and coronary plaque burden and the number of noncalcified plaques in subjects undergoing CT angiography [11]. However, in some studies, serum chemerin levels have not been demonstrated to be related with the atherosclerotic plaque burden [25]. Herein, we could not find a significant relationship between the serum chemerin levels and extent of coronary artery disease; however, serum chemerin levels were significantly correlated with inflammatory markers and coronary thrombus burden.

In our study, one of the important findings was the relationship between serum CRP and chemerin levels. This finding may be a clue that chemerin is an important factor in the inflammatory process of acute STEMI. Increased CRP levels as a marker of inflammation have been shown to be related with increased morbidity and mortality in STEMI patients [26]. The relationship between high thrombus burden and CRP levels which has been recently shown by Niccoli et al. [27] is one of the explanations of this relationship. Herein, we have also found increased CRP levels in patients with thrombus burden. Besides, serum chemerin levels were also increased in these patients, and after multivariate analysis, this finding still persisted.

In our study, we have also found that higher serum chemerin levels were associated with higher peak serum CK-MB levels as a marker of increased necrotized myocardial tissue. Increased thrombus burden in patients with high chemerin levels may explain this finding, because in these patients, post-PCI TIMI frame counts were higher showing poorer perfusion of the jeopardized myocardial tissue possibly due to distal embolization of the increased thrombus burden.

In the pathogenesis of coronary artery disease and acute coronary syndromes, endothelial dysfunction is another important factor precipitating the development of atherosclerosis. The endothelium is the major regulator of vascular homeostasis and maintains the balance between vasodilatation and vasoconstriction, stimulation and inhibition of smooth muscle proliferation and migration, and thrombogenesis and fibrinolysis [28]. As we know, chemerin induces insulin resistance which is associated with endothelial dysfunction and inflammation in primary human skeletal muscle cells [29]. It was also shown that insulin resistance is associated with endothelial dysfunction in both peripheral arteries and coronary arteries [29]. Therefore, we can say that increased chemerin levels may cause more endothelial dysfunction resulting in increased thrombus formation and worse coronary arterial flow assessed by TIMI frame count in our study.

4.1. Study Limitations

The present study was cross sectional and collected data at one point in time, thus not allowing for inferences or changes over time. Another limitation of our study was the low number of patients. No significant clinical event developed in the hospitalization period of the patients, and therefore we could not assess the clinical significance of this newly discovered molecule. Besides, long-term follow-up of the patients were missing, because inclusion of patients has recently ended. A molecule which may predict the long-term clinical manifestations of acute STEMI would have been more valuable.

The strengths of our study were as follows: (a) a recently discovered molecule, chemerin, has been studied in a selected population, that is, STEMI patients undergoing primary PCI, and (b) plasma chemerin levels were firstly discovered to be related to the coronary thrombus burden in the setting of STEMI which might lead further studies and new treatment strategies targeting chemerin.

5. Conclusion

Results from our study have demonstrated for the first time that chemerin levels were higher in STEMI patients with greater thrombus burden and higher level of inflammation. The main role of chemerin in the pathogenesis of acute STEMI should be investigated by further studies.

Disclosure

An earlier version of this work was presented as a poster with the title “Plasma chemerin levels were increased in ST elevation myocardial infarct patients with high thrombus burden” at 33rd International Congress of Turkish Cardiology 2017.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

References

  1. P. E. Scherer, “Adipose tissue: from lipid storage compartment to endocrine organ,” Diabetes, vol. 55, no. 6, pp. 1537–1545, 2006. View at Publisher · View at Google Scholar · View at Scopus
  2. R. S. Ahima and J. S. Flier, “Adipose tissue as an endocrine organ,” Trends in Endocrinology and Metabolism, vol. 11, no. 8, pp. 327–332, 2000. View at Publisher · View at Google Scholar · View at Scopus
  3. P. Trayhurn and I. S. Wood, “Adipokines: inflammation and the pleiotropic role of white adipose tissue,” British Journal of Nutrition, vol. 92, no. 3, pp. 347–355, 2004. View at Publisher · View at Google Scholar · View at Scopus
  4. B. B. Kahn and J. S. Flier, “Obesity and insulin resistance,” Journal of Clinical Investigation, vol. 106, no. 4, pp. 473–481, 2000. View at Publisher · View at Google Scholar · View at Scopus
  5. S. Nagpal, S. Patel, H. Jacobe et al., “Tazarotene-induced gene 2 (TIG2), a novel retinoid-responsive gene in skin,” Journal of Investigative Dermatology, vol. 109, no. 1, pp. 91–95, 1997. View at Publisher · View at Google Scholar · View at Scopus
  6. A. A. Roman, S. D. Parlee, and C. J. Sinal, “Chemerin: a potential endocrine link between obesity and type 2 diabetes,” Endocrine, vol. 42, no. 2, pp. 243–251, 2012. View at Publisher · View at Google Scholar · View at Scopus
  7. C. Kutzleb, A. Busmann, M. Wendland, and E. Maronde, “Discovery of novel regulatory peptides by reverse pharmacology; spotlight on chemerin and the RF-amide peptides metastin and QRFP,” Current Protein and Peptide Science, vol. 6, no. 3, pp. 265–278, 2005. View at Publisher · View at Google Scholar · View at Scopus
  8. K. B. Goralski, T. C. McCarthy, E. A. Hanniman et al., “Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism,” Journal of Biological Chemistry, vol. 282, no. 38, pp. 28175–28188, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. V. Wittamer, B. Bondue, A. Guillabert, G. Vassart, M. Parmentier, and D. Communi, “Neutrophil-mediated maturation of chemerin: a link between innate and adaptive immunity,” Journal of Immunology, vol. 175, no. 1, pp. 487–493, 2005. View at Publisher · View at Google Scholar · View at Scopus
  10. J. Weigert, M. Neumeier, J. Wanninger et al., “Systemic chemerin is related to inflammation rather than obesity in type 2 diabetes,” Clinical Endocrinology, vol. 72, no. 3, pp. 342–348, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. M. Lehrke, A. Becker, M. Greif et al., “Chemerin is associated with markers of inflammation and components of the metabolic syndrome but does not predict coronary atherosclerosis,” European Journal of Endocrinology, vol. 161, no. 2, pp. 339–344, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Inci, G. Aksan, and P. Dogan, “Chemerin as an independent predictor of cardiovascular event risk,” Therapeutic Advances in Endocrinology and Metabolism, vol. 7, no. 2, pp. 57–68, 2016. View at Publisher · View at Google Scholar · View at Scopus
  13. S. G. Spiroglou, C. G. Kostopoulos, J. N. Varakis, and H. H. Papadaki, “Adipokines in periaortic and epicardial adipose tissue: differential expression and relation to atherosclerosis,” Journal of Atherosclerosis and Thrombosis, vol. 17, no. 2, pp. 115–130, 2010. View at Publisher · View at Google Scholar
  14. G. G. Gensini, “A more meaningful scoring system for determining the severity of coronary heart disease,” American Journal of Cardiology, vol. 51, no. 3, p. 606, 1983. View at Publisher · View at Google Scholar · View at Scopus
  15. G. Sianos, M. I. Papafaklis, and P. W. Serruys, “Angiographic thrombus burden classification in patients with ST-segment elevation myocardial infarction treated with percutaneous coronary intervention,” Journal of Invasive Cardiology, vol. 22, no. 10, pp. 6B–14B, 2010. View at Google Scholar
  16. O. Topaz, “Comparison between thrombus removal devices: aspirations meet reality,” Catheterization and Cardiovascular Interventions, vol. 78, no. 1, pp. 20–22, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. C. M. Gibson, J. A. de Lemos, S. A. Murphy et al., “Combination therapy with abciximab reduces angiographically evident thrombus in acute myocardial infarction: a TIMI 14 substudy,” Circulation, vol. 103, no. 21, pp. 2550–2554, 2001. View at Publisher · View at Google Scholar · View at Scopus
  18. A. Tedgui and Z. Mallat, “Cytokines in atherosclerosis: pathogenic and regulatory pathways,” Physiological Reviews, vol. 86, no. 2, pp. 515–581, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. Q. Wan, X. Cui, J. Shao et al., “Beijing ambient particle exposure accelerates atherosclerosis in ApoE knockout mice by upregulating visfatin expression,” Cell Stress and Chaperones, vol. 19, no. 5, pp. 715–724, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. S. E. Alam, S. S. Nasser, K. E. Fernainy, A. A. Habib, and K. F. Badr, “Cytokine imbalance in acute coronary syndrome,” Current Opinion in Pharmacology, vol. 4, no. 2, pp. 166–170, 2004. View at Publisher · View at Google Scholar · View at Scopus
  21. C. L. Huang, Y. W. Wu, C. C. Wu et al., “Association between serum adipocyte fatty-acid binding protein concentrations, left ventricular function and myocardial perfusion abnormalities in patients with coronary artery disease,” Cardiovascular Diabetology, vol. 12, no. 1, p. 105, 2013. View at Publisher · View at Google Scholar · View at Scopus
  22. Y. Nakamura, K. Shimada, D. Fukuda et al., “Implications of plasma concentrations of adiponectin in patients with coronary artery disease,” Heart, vol. 90, no. 5, pp. 528–533, 2004. View at Publisher · View at Google Scholar
  23. T. Pischon, C. M. Bamberger, J. Kratzsch et al., “Association of plasma resistin levels with coronary heart disease in women,” Obesity Research, vol. 13, no. 10, pp. 1764–1771, 2005. View at Publisher · View at Google Scholar · View at Scopus
  24. H. J. Yoo, H. Y. Choi, S. J. Yang et al., “Circulating chemerin level is independently correlated with arterial stiffness,” Journal of Atherosclerosis and Thrombosis, vol. 19, no. 1, pp. 59–66, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. K. Aydin, U. Canpolat, S. Akin et al., “Chemerin is not associated with subclinical atherosclerosis markers in prediabetes and diabetes,” Anatolian Journal of Cardiology, vol. 16, pp. 749–755, 2016. View at Publisher · View at Google Scholar · View at Scopus
  26. T. Trepels, A. M. Zeiher, and S. Fichtlscherer, “Acute coronary syndrome and inflammation. Biomarkers for diagnostics and risk stratification,” Herz, vol. 29, no. 8, pp. 769–776, 2004. View at Publisher · View at Google Scholar · View at Scopus
  27. G. Niccoli, A. Menozzi, D. Capodanno et al., “Relationship between serum inflammatory biomarkers and thrombus characteristics in patients with ST segment elevation myocardial infarction,” Cardiology, vol. 137, no. 1, pp. 27–35, 2017. View at Publisher · View at Google Scholar · View at Scopus
  28. T. F. Luscher and M. Barton, “Biology of the endothelium,” Clinical Cardiology, vol. 20, no. 11, p. II-3-10, 1997. View at Google Scholar
  29. A. Sjoholm and T. Nystrom, “Endothelial inflammation in insulin resistance,” The Lancet, vol. 365, no. 9459, pp. 610–612, 2005. View at Publisher · View at Google Scholar