Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs

Figure 2

Omentin-1 reduced the PA-induced attachment of THP-1 cells to HUVECs. ICAM-1 and MCP-1 protein expression levels in HUVECs from different groups were detected with western blotting (a) using tubulin or GAPDH as loading control. Quantitative analysis of protein expression is shown as bar graphs. The mRNA expression levels of ICAM-1 and MCP-1 were determined with RT-qPCR (b). (c, d) Representative images of cellular fluorescence assay for ICAM-1 and MCP-1 (400x magnification) and the quantitative analysis of fluorescence intensity per cell. (e) The representative pictures of BCECF-AM-labeled THP-1 cells attached to HUVECs in different treatments groups are shown (400x magnification); the number of fluorescent cells was counted. The values are mean ± SEM of three independent experiments. vs. PA group, vs. PA group, and vs. PA group.

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