Upregulation of MicroRNA-125b Leads to the Resistance to Inflammatory Injury in Endothelial Progenitor Cells
Upregulation of miR-125b in EPCs attenuated the expression of proinflammatory factors and ameliorated the TNF-α-induced cell apoptosis. EPCs were treated with TNF-α (10 ng/mL) for 1 h after transfection. The mRNA levels of proinflammatory factors (TNF-α, IL-1β, and IL-6) were measured by qRT-PCR (a). Cell apoptosis was determined by flow cytometry using annexin V staining (b). The activation of caspase3 was analyzed by western blotting. Representative (c) and quantification (d) of caspase3 level (normalized to β-actin). The activation of NF-κB was determined by the level of p-p65 in EPCs using western blotting. Representative (e) and quantification (f) of p-p65 level (normalized to p65) ( vs. NC mimic with TNF-α treatment, n = 5).