Functional Genetic Variant in ATG5 Gene Promoter in Acute Myocardial Infarction

Figure 3

Relative transcriptional activity of wild-type and variant ATG5 gene promoters. Wild-type and variant ATG5 gene promoters were cloned into the reporter gene vector pGL3 and transfected into cultured cells. The transfected cells were collected, and dual-luciferase activities were assayed. Empty vector pGL3-basic was used as a negative control. Transcriptional acitivity of the wild-type ATG5 gene promoter was designed as 100%. Relative activities of ATG5 gene promoters were calculated. (a). Relative activities of wild-type and variant ATG5 gene promoters in HEK-293 cells. (b). Relative activities of wild-type and variant ATG5 gene promoters in H9c2 cells. Lanes 1, pGL3-basic; 2, pGL3-WT; 3, pGL3-106326168_70delTCT; 4, pGL3-g.106326293T; 5, pGL3-106325913C; 6, pGL3-106325876T, and 7, pGL3-106326155C. , .

(a)

(b)